Naslov
Biorelevant dissolution of biologically active components from herbal tablets used in the treatment of inflammatory bowel diseases

Autori
Zubčić, Snježana ; Kovačić, Jelena ; Jeličić, Mario- Livio ; Klarić, Ilija ; Mornar, Ana ; Tomić, Siniša ; Amidžić Klarić, Daniela

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
7th PhD Student Symposium, Book of abstracts / Pavlek, Katarina - Zagreb : Prirodoslovno-matematički fakultet Sveučilišta u Zagrebu, 2023, 130-130

ISBN
978-953-6076-01-7

Skup
7th PhD Student Symposium

Mjesto i datum
Zagreb, Hrvatska, 21.04.2023. - 22.04.2023

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
biorelevant media, herbal tablet, dietary supplements, inflammatory bowel diseases, HPLC-DAD

Sažetak
Inflammatory bowel disease (IBD) is a continuously increasing worldwide disease and affects people of all ages. More than half of these patients use herbal dietary supplements to alleviate the symptoms of IBD [1]. Curcuminoid compounds present in turmeric, boswellic acids as pentacyclic terpenoid molecules from frankincense resin, andrographolides as diterpene lactones isolated from green chiretta, and piperine as a piperidine alkaloid from black pepper fruits are the most abundant biologically active substances in herbal dietary supplements for the treatment of IBD. Since today the use of dietary supplements is widespread and constantly increasing, this study aimed to analyze the dissolution of those mentioned biologically active components from oral herbal preparations in biorelevant media. Samples in tablet dosage form were collected from local pharmacies and purchased from the Internet. All dietary supplements were classified as botanical monopreparations and multipreparations. In vitro dissolution studies were conducted using a USP dissolution (paddle) apparatus 2 (Erweka DT600, Erweka GmbH, Langen, Germany). The herbal tablets were dispersed in 900 mL of USP- recommended dissolution medium (1 % sodium lauryl sulfate), fasted/fed simulated gastric and small intestinal fluids (FaSSGF, FaSSIF/FeSSIF) at 37 °C [2]. During dissolution, 1 mL of sample was collected at each time point up to 120 min and filtered through Minisart RC4, 0.45 μ m filters (Sartorius, Germany), which did not retain any of the analytes. All samples were analyzed in duplicate. The concentration of biologically active components in the filtrate was analyzed using an HPLC-DAD assay. Chromatographic analyses were performed on a Shimadzu Nexera chromatographic system. Based on the obtained results (similarity factor, f2 value < 50 %), the in vitro release profile of the biologically active substance from herbal tablets depends on the composition of the biorelevant medium.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Javno zdravstvo i zdravstvena zaštita, Farmacija

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