Naslov
Impurity characterization of spingosine-1- phosphate (S1P) modulator etrasimod using liquid chromatography-mass spectrometry (LC-MS/MS)
(Impurity characterization of spingosine-1- phosphate (S1P) modulator etrasimod using liquid chromatography-mass spectrometry (LC-MS/MS) (P- CH19))

Autori
Kovačić, Jelena ; Jeličić, Mario-Livio ; Amidžić Klarić, Daniela ; Mornar, Ana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
7th FACULTY OF SCIENCE PhD STUDENT SYMPOSIUM-BOOK OF ABSTRACTS / Pavlek, Katarina - Zagreb : Prirodoslovno-matematički fakultet Sveučilišta u Zagrebu, 2023, 122-122

ISBN
978-953-6076-01-7

Skup
7th FACULTY OF SCIENCE PhD STUDENT SYMPOSIUM

Mjesto i datum
Zagreb, Hrvatska, 21.04.2023. - 22.04.2023

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
IBD ; etrasimod ; HPLC

Sažetak
Inflammatory Bowel Disease (IBD), Crohn's disease and ulcerative colitis (UC) are chronic and progressive diseases. Their etiology is not fully understood, and it is thought to involve a combination of genetic and environmental factors leading to an imbalance between pro-inflammatory and anti- inflammatory cytokines in the gastrointestinal tract. Sphingosine-1-phosphate (S1P) receptor modulators represent a new generation of drugs that will make an important turning point in the treatment of IBD [1]. Etrasimod is a second generation S1P receptor modulator intended specifically for the treatment of IBD. Phase II clinical trials in patients with moderate to severe UC in 2019 showed good efficacy of the drug, and it is currently in phase III clinical trials [2]. In this research we developed high-performance liquid chromatography coupled with electrospray ionization triple-quadrupole tandem mass spectrometry (LC-QqQMS/MS) method for quantification of etrasimod and its impurities. The chromatographic analysis of etrasimod was performed on a 10 cm Poroshell 120 column with a gradient mobile phase of ultra-pure water and acetonitrile with the addition of formic acid (0.1 %) as a modifier at a flow rate of 0.8 mL/min in 16 min. Total ion chromatogram (TIC) of product ion showed showed a visible peak of etrasimod with impurities that will be confirmed by forced degradation studies. As a result, this development of analytical methods will be used to test the stability of etrasimod and the compatibility of the drug and excipients.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Farmacija

POVEZANOST RADA