Naslov
Is immunophenotyping a useful in diagnosis of Atopic dermatitis diagnosis?

Autori
Lipozenčić, Jasna ; Jakić-Razumović, Jasminka ; Ljubojević, Suzana ; Bobek, Dubravka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, stručni

Izvornik
Abstract Book on International Symposium on Atopic Dermatitis / Giannetti, A ; Ring, Johannes ; Taieb, A ; Thestrup-Pederson K - Rim : Medical Communications Srl, 2003

Skup
International Symposium on Atopic Dermatitis, Third Georg Rajka Symposium

Mjesto i datum
Rim, Italija, 29.08.2003. - 31.08.2003

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Imunophenotyping; atopic dermtitis diagnosis; in vivo and in vitro diagnostic tools

Sažetak
Introduction. There are many in vitro and in vivo diagnostic tools to perform immunological dysfunction in the pathogenesis of AD. Immunophenotyping of inflammatory cell surface markers in the biopsies of AD lesions was made. Methods. To perform the presence of inflammatory cell surface markers in the biopsies of skin lesions we performed immunohistochemical analysis in 15 AD patients and 5 healthy subjects. Standard avidin-biotin immunoperoxidase staining of paraphin-embadded, 4 μ m skin sections with semiquantitative counting of cells labeled with anti-CD3, anti CD8, anti CD20, anti-HLA DR (HLA human leukocyte antigen), and anti -immunoglobulin E (anti-IgE) primary antibodies, was done. Results. The results of our study showed that CD4+ prevail over CD8+ in the infiltrate of AD skin lesions. In comparison with normal skin, a higher expression of HLA DR on dermal lymphocytes and epidermal LCs and IgE + cells were found. The infiltrating cell expressing CD3, CD4 and HLA-DR cell-surface markers in the dermis and epidermis, CD8 in the dermis and IgE+ cells in the epidermis. Conclusion. Immunohistochemistry has recently become a recognized and necessary part of pathodiagnosis in dermatopathology. The comparison of the AD patient group and the control group pf healthy subjects showed that the two groups differed in the number of infiltrating cells expressing CD3, CD4 and HLA-DR cell surface markers in the dermis and epidermis, CD8 in the dermis and IgE+ cells in the epidermis.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

POVEZANOST RADA