Naslov
Biflavonoid profiling in different tissues of ginkgo (𝘎𝘪𝘯𝘬𝘨𝘰 𝘣𝘪𝘭𝘰𝘣𝘢 L.)

Autori
Jurčević, Iva ; Kovač Tomas, Marija ; Šamec, Dunja

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
7th FACULTY OF SCIENCE PhD STUDENT SYMPOSIUM BOOK OF ABSTRACTS / Pavlek, Katarina - Zagreb : Prirodoslovno-matematički fakultet Sveučilišta u Zagrebu, 2023, 91-91

ISBN
978-953-6076-01-7

Skup
7th PhD Student Symposium

Mjesto i datum
Zagreb, Hrvatska, 21.04.2023. - 22.04.2023

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
amentoflavone ; bilobetin ; biflavonoids ; ginkgo ; ginkgetin ; HPLC-DAD ; isoginkgetin ; sciadopitysin ; tissue specific profiling

Sažetak
Flavonoids are the best-studied group of specialized plant metabolites important for a wide range of plant interactions with the environment. After many years of science focusing on the monomeric forms of flavonoids, researched have been shifted to better understanding of the less studied dimeric forms, biflavonoids [1]. Structurally, biflavonoids are polyphenolic compounds consisting of two identical or non- identical flavone-flavonoid units linked in a symmetrical or asymmetrical manner by an alkyl or alkoxy linker of different lengths. They are restricted to certain species in the plant kingdom and one of the plants rich in biflavonoids is ginkgo (𝘎𝘪𝘯𝘬𝘨𝘰 𝘣𝘪𝘭𝘰𝘣𝘢 L.) [2, 3]. Although ginkgo is a well-known medicinal plant, the tissue-specific distribution of biflavonoids in ginkgo has not yet been elucidated. Therefore, the aim of our study was to develop a HPLC-DAD method for the simultaneous determination of five biflavonoids (amentoflavone, bilobetin, ginkgetin, isoginkgetin, and sciadopitysin) in 10 different ginkgo tissue types. We measured the total content of polyphenols and flavonoids using spectrophotometric methods as well. We detected biflavonoids only in the plant parts that are in direct contact with the environment (leaves - petioles and leaf blades, tree and branch bark, buds, seed stalks, and sarcotesta), which may indicate their role in plant-environment interaction. The highest content of biflavonoids was found in leaves (7090 ± 35.75 μg/g dw), with sciadopitysin (2398 ± 727.40 μg/g dw) being the predominant compound. In contrast, in tree bark and twigs, the most abundant biflavonoid was amentoflavone (63.34 ± 4.57 μg/g dw and 75.70 ± 6.80, respectively) [4]. Our results showed a different distribution of methylated biflavonoids through the tissues when compared to their unmethylated form, which might indicate their different roles in plants, but their exact biological functions remain to be explored.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Prehrambena tehnologija

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