Naslov
SERS spectra of berberine and papaverine

Autori
Petrović, Petra ; Tikvenjak, Patricia ; Miljanić, Snežana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
28. HSKIKI BOOK OF ABSTRACTS / Rogošić, Marko - Zagreb : Hrvatsko društvo kemijskih inženjera i tehnologa (HDKI), 2023, 115-115

Skup
28th Croatian Meeting of Chemists and Chemical Engineers

Mjesto i datum
Rovinj, Hrvatska, 28.03.2023. - 31.03.2023

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Berberine ; Papaverine ; SERS ; nanoparticles

Sažetak
Surface-enhanced Raman scattering (SERS) spectroscopy is a powerful analytical technique that allows detection of molecules at very low concentrations by amplifying the Raman scattering signal due to adsorption or chemical binding of molecules to a nanostructured metal surface. In this study, the enhancement of Raman scattering of two plant alkaloids, berberine and papaverine, was investigated. Alkaloids are natural compounds, mainly secondary metabolites of plants, which have strong pharmacological activity and have been used for centuries in traditional, and nowadays in modern medicine. SERS spectra of berberine and papaverine were obtained using citrate-stabilized silver nanoparticles and a 785 nm laser excitation source. Characteristic vibrational bands of berberine and papaverine were observed and assigned. The addition of aggregating agent (0.1 M MgSO4) into the silver colloid resulted in a significant increase of the SERS intensity, allowing detection of both alkaloids in nanomolar range. Nevertheless, a nonlinear relationship between concentration and SERS intensity was observed in the concentration dependent spectra (5×10-9 - 1×10-4 M) as a result of the surface selection rules. Moreover, the SERS spectra of papaverine in the low concentration range (< 1×10- 6 M) were similar to the papaverine spectrum (1×10-4 M) in the acidic medium (pH = 1.2), indicating that due to electrostatic interactions with the stabilizing citrate anions on the metallic surface only protonated molecules (pKa = 6.4) contributed to the spectra measured at low papaverine concentrations in the neutral colloidal suspension (pH = 7.0). This study demonstrated that the SERS spectroscopy can be successfully used as a sensitive technique for detecting and identifying alkaloids.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

POVEZANOST RADA