Naslov
Development and optimization of biflavonoids extraction from ginkgo (Ginkgo biloba L.) leaves using deep eutectic solvents

Autori
Bajo, Matea ; Radović, Mia ; Cvjetko Bubalo, Marina ; Jurinjak Tušek, Ana ; Šalić, Anita ; Zelić, Bruno ; Šamec, Dunja

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
28HSKIKI Book of Abstracts / Rogošić, Marko - Zagreb : Hrvatsko društvo kemijskih inženjera i tehnologa (HDKI), 2023, 144-144

Skup
28th Croatian Meeting of Chemists and Chemical Engineers

Mjesto i datum
Rovinj, Hrvatska, 28.03.2023. - 31.03.2023

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
biflavonoids ; COSMOtherm ; natural deep eutectic solvents ; extraction

Sažetak
The plant kingdom is a known reservoir of bioactive natural products. The best-studied group to date is flavonoids, which perform multiple functions in plants and have a major impact on human health. Flavonoids can occur in free form in plants, but are more commonly glycosylated, methylated, acetylated, prenylated, or polymerized, which affects their bioactivity. Flavonoid dimers, known as biflavonoids, which consist of two identical or different flavonoid units, are a less-studied subgroup of flavonoids but have recently been recognized as potential pharmaceutical agents, particularly because of their antiviral, antifungal, and other bioactivities. They are commonly found in plants used in traditional medicine, such as ginkgo (Ginkgo biloba L.). The crucial step for the pharmaceutical use of any natural product is its isolation from the natural matrix. This process usually involves the use of various organic solvents, which can be hazardous to the environment and humans. Therefore, natural deep eutectic solvents (NADES) have recently been recognized as environmentally friendly and cheap extraction agents, whose additional advantage is their pronounced flexibility. To select the best NADES for efficient extraction of biflavonoids from ginkgo, COSMOtherm software was used and 288 NADES were studied. Based on the obtained model predictions, 15 NADES were selected, synthesized and characterized (density, viscosity, pH). After synthesis, the extraction of biflavonoids was performed in a series of batch experiments with the same initial mass of crushed leaves of ginkgo, where the extraction efficiency was defined as a criterion for the selection of the best NADES (Fig. 1). The highest extraction efficiency was obtained with Choline Chloride : Ethylene Glycol (ChCl:EG) 1:2 NADES containing 20% H2O. Compared to extraction with 70% methanol, 13% higher extraction efficiency was obtained using ChCl:EG 1:2 with 20% H2O NADES under the same process conditions. The extraction with selected process was further optimized using the experimental Box- Behnken design at three levels with three factors: extraction time, ratio of initial mass of shredded leaves and mass of NADES, and temperature.

Izvorni jezik
Engleski

Znanstvena područja
Kemijsko inženjerstvo, Biotehnologija, Prehrambena tehnologija

POVEZANOST RADA