Naslov
ELEVATED C MAZE - TO TEST OR NOT TO TEST IN THE MORNING?

Autori
Tvrdeić, Ante ; Miše, Branko ; Babacanli, Alen ; Poljak, Ljiljana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
10th Croatian Congress of Pharmacology and the 1st Croatian Congress of Clinical Pharmacology with International Participation, Abstract Book / Mršić-Pelčić, Jasenka ; Vitezić, Dinko ; Janković, Tamara - Zagreb : Zavod za kliničku farmakologiju Klinika za unutarnje bolesti KBC Zagreb- Rebro, 2022, 158-158

Skup
10. hrvatski kongres farmakologije ; 1. hrvatski kongres kliničke farmakologije s međunarodnim sudjelovanjem = 10th Croatian Congress of Pharmacology ; 1st Croatian Congress of Clinical Pharmacology and Therapeutics with International Participation

Mjesto i datum
Opatija, Hrvatska, 22.09.2022. - 25.09.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
Elevated C maze ; anxiety ; test time

Sažetak
To find out whether different testing periods during an active (dark) phase of the day might modulate anxiety-like behaviour in an elevated C maze (ECM), we assigned 30 male Wistar rats to groups of 10 animals tested in ECM during the morning (10-12 hrs), early afternoon (14-16 hrs) or late afternoon (18-20 hrs). ECM have the shape of the letter C, it is 80 cm elevated from the floor, enclosed in the middle third (22 cm long, 15 cm wide) with two side walls of 30 cm in height. Two-thirds of the maze (each 22 x15 cm) are open, without walls. For testing, individual rats were allowed to freely explore ECM for 5 minutes. Behaviour data were collected and analysed by camera and video tracking software Any maze 4.82. Results showed that independent of testing time, rats had a significantly higher number of entrances, spend more time and travel a longer distance in the safe, enclosed zone of ECM as compared to unsafe open zones. Also, we found no differences in horizontal or vertical rat locomotion in ECM or ECM zones. But freezing time (defensive behaviour) was longer in the morning and early afternoon than in the late afternoon for ECM. In the enclosed ECM zone, the number of faecal boli (a sign of emotionality), the number of head dipping (exploration) and the number of entrances (avoidance) were lower during the morning and early afternoon testing period. In conclusion, testing of Wistar rats during different periods within the active (dark) phase of the day might modulate anxiety-like behaviour in ECM.

Izvorni jezik
Engleski

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