Naslov
NO FEAR TO EXPLORE THE UNSAFE WITH CEFTRIAXONE!

Autori
Tvrdeić, Ante ; Miše, Branko ; Babacanli, Alen ; Ljiljana Poljak

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
10th Croatian Congress of Pharmacology and the 1st Croatian Congress of Clinical Pharmacology with International Participation, Abstract Book Opatija, September 22 – 25, 2022 / Mršić-Pelčić, Jasenka ; Vitezić, Dinko ; Janković, Tamara - Zagreb : Zavod za kliničku farmakologiju Klinika za unutarnje bolesti KBC Zagreb- Rebro, 2022, 157-157

Skup
10. hrvatski kongres farmakologije ; 1. hrvatski kongres kliničke farmakologije s međunarodnim sudjelovanjem = 10th Croatian Congress of Pharmacology ; 1st Croatian Congress of Clinical Pharmacology and Therapeutics with International Participation

Mjesto i datum
Opatija, Hrvatska, 22.09.2022. - 25.09.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
Ceftriaxone, anxiety, elevated plus maze, elevated T-maze

Sažetak
Antibiotic ceftriaxone enhances the expression of GLT1 glutamate membrane transporter in the brain and decreases the availability of synaptic glutamate. To investigate whether this anti glutamate effect of ceftriaxone could modulate anxiety, we used elevated plus maze (EPM) and elevated T maze (ETM) tests. EPM is a plus sign shape elevated 60 cm from the floor with two opposite enclosed arms (50x10 cm with 30 cm high walls) vertical to two opposite open arms (50x10 cm) and intersection zone (10x10 cm). The individual rat was left in the intersection zone to investigate EPM for 5 minutes. Avoidance of open, unsafe arms in EPM suggests anxiety. ETM is the same as EPM with one blocked enclosed arm. ETM measure avoidance behaviour in 4 subsequent avoidance sessions and escape behaviour in 4 escape sessions. In the avoidance ETM session, the rat starts at the distal end of the enclosed arms. But in the escape ETM session at the distal end of the open arms. Both avoidance and escape individual sessions last 5 minutes. We assigned 21 or 22 male Wistar rats for ETM or EPM in groups treated with ceftriaxone (200 mg/kg IP., 1 x daily in 9 days) or saline (5mL/kg IP.). Behavioural data were collected and analysed by camera and video tracking software Any maze 4.82. Results revealed that ceftriaxone significantly increases exploration behaviour (number of head dipping) in unsafe zones of EPM (open arms and intersection zone) without influencing avoidance, escape, locomotion or defensive behaviour in EPM or ETM.

Izvorni jezik
Engleski

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