Naslov
High resolution microfluidic microchip electrophoresis for first separation and quantification of amino acids in complex dietary supplements

Autori
Jozanović, Marija ; Pukleš, Iva ; Matasović, Brunisalv ; Jakić, Marija ; Sakač, Nikola

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, ostalo, znanstveni

Skup
World Congress Food Safety and Nutrition

Mjesto i datum
Rim, Italija, 27.04.2021. - 28.04.2021

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Supplements, MCE

Sažetak
In modern nutrition research amino acids and peptides have a broad potential therapeutic applications, and are used as supplements or as functional food ingredients. β-alanine, L- histidine and carnosine are common used in the form of dietary supplements. Carnosine is dipeptide produced by condensation of β-alanine and L-histidine which is the reason these two amino acids are often used simultaneously. Standard methods for analysis of amino acid and peptides have a number of disadvantages. They are expensive, complex and time consuming. Microchip electrophoresis evolved from capillary electrophoresis with purpose of reducing the time and cost of analyzes, the amount of reagents, samples and waste. During the study various parameters was investigated to provide high resolution and to optimize the separation. A key point was to optimize the separation buffer in order to avoid overlapping of the amino acids with the other constituents in the analyzed sample. Linear response region for all three analytes was determined using linear regression. Detection limits were below 1 mg/mL. Proposed microfluidic methods are environmentally friendly and offer great promises for routine multi-analyte pharmaceuticals analyses.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Kemijsko inženjerstvo

POVEZANOST RADA