Naslov
Tissue microRNAs in prostate cancer - (pre)analytical challenges

Autori
Dobrović, Irena ; Ulamec, Monika ; Vrtarić, Alen ; Pezelj, Ivan ; Sinčić, Nino

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, stručni

Izvornik
27th Ljudevit Jurak International symposium on comparative pathology with one health session : final program & book of abstracts / - , 2019, 51-52

Skup
27th Ljudevit Jurak International symposium on comparative pathology with one health session

Mjesto i datum
Zagreb, Hrvatska, 31.05.2019. - 01.06.2019

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
prostate cancer ; biomarkers ; miRNAs ; FFPE

Sažetak
With prostate cancer (PCa) being the most commonly diagnosed neoplasia among men all age, according Globocan, there is a need for more sophisticated biomarkers for diagnosis, prognosis and management. MicroRNAs (miRNAs) have emerged as potential biomarkers for PCa that could allow its distinguishing from benign prostate hyperplasia, as well as aggressive from non-aggressive PCa. Even though it seems that the biggest miRNAs potential lies in their use as noninvasive biomarkers in liquid biopsies, research is mostly based on miRNAs in PCa tissue obtained by biopsy, both formalin-fixed paraffin- embedded (FFPE) and fresh frozen. However, questions are being raised regarding diverse (pre)analytical factors that influence miRNA analysis, such as FFPE block age. By examining published papers, we would like to present current knowledge and challenges in this area. Preanalytical variables in research of miRNAs in FFPE tissue include time ex vivo, fixative, storage, microdissection and immunochemistry. Among those, FFPE storage represents a factor that highly differs between studies since blocks stored for different time periods were used in PCa research. Moreover, conflicting data was published regarding miRNAs stability in FFPE from different tissues. Some studies did not find significant change in miRNA levels depending on FFPE sample age, while others declared notable decrease in detected miRNA depending on sample storage time. Study of the effect of long-term storage on PCa FFPE tissue demonstrated significant loss of miRNA stability and suggested using sample block age rather than RNA quality when adjusting data. It also seems that FFPE tissue storage affects differently specific miRNAs, with some being more stable than others over the years. However, majority of studies on PCa FFPE tissue did not report how long were their samples stored. With raising research on miRNAs in PCa, there is a need for standardization of analytical procedures in order to be able to compare data from different studies. One of many aspects of this problem is FFPE PCa tissue storage which requires wildly excepted guidelines on storage conditions. For now, it seems advisable to consider adjusting data when analyzing miRNAs in FFPE samples stored for more than a decade.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA