Naslov
Moderately increased temperature modifies heat stress response in BPM1-overexpressing thale cress (Arabidopsis thaliana (L.) Heynh.)

Autori
Kutnjak, Marin ; Vuković, Matea ; Vitko, Sandra ; Vidaković-Cifrek, Željka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Knjiga sažetaka Simpozija studenata bioloških usmjerenja 2022. / Hus, Petra ; Milišić Bogunović, Ema ; Cahunek, Iva ; Španić, Filip ; Tomić, Dominik - Zagreb : Udruga studenata biologije, 2022, 62-63

Skup
Simpozij studenata bioloških usmjerenja 2022.

Mjesto i datum
Zagreb, Hrvatska, 21.05.2022. - 22.05.2022

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Domaća recenzija

Ključne riječi
heat stress, oxidative stress, priming, BPM1 gene, Arabidopsis thaliana

Sažetak
Increased temperature is an environmental stressor to which plants are exposed, causing heat stress through a series of changes in plant cells. Resistance to future stress conditions can be improved by applying more moderate stress before exposure to severe stress, i.e. by priming. This study aimed to determine the effect of priming on oxidative stress and antioxidative response (by H2O2 level, lipid peroxidation level, proline content, and antioxidant enzymes activity measurements), HSP70 protein expression (by western blotting), and photosynthetic efficiency (by OJIP method). The research was performed on wild-type Arabidopsis thaliana (L.) Heynh. seedlings and BPM1-overexpressing line (oeBPM1). One group of plants was exposed to an increased temperature of 42 °C for six hours, another group to 42 °C after priming at a temperature of 37 °C, while the third, control group of plants, was not exposed to an increased temperature. Priming led to better long-term survival after heat stress in plants. Exposure to heat stress led to the induction of antioxidant enzymes activity, increased HSP70 protein expression, and significant disruption of photosynthetic efficiency in cells. The observed lipid peroxidation level and the activity of catalase and ascorbate peroxidase indicate that the oeBPM1 line has a partially better response to heat stress than wild-type plants, suggesting possible roles of the BPM1 protein. Finally, the results of this study showed that overexpression of the BPM1 gene in A. thaliana and priming have an impact on the heat stress response, raising many questions for future research on the BPM1 gene and potential applications of priming in plants.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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