Naslov
Bypassing Occlusion: Abdominal Aorta Occlusion in Rats and the Therapy with the Stable Gastric Pentadecapeptide BPC 157

Autori
Kasnik, Katarina ; Kralj, Tamara ; Pavlov, Katarina Horvat ; Drmic, Domagoj ; Blagaic, Alenka Boban ; Seiwerth, Sven ; Sikiric, Predrag

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
FASEB Journal. 2018 ; 32(S1) / - : John Wiley & Sons, 2018, 69913-69913

Skup
Experimental Biology

Mjesto i datum
San Diego (CA), Sjedinjene Američke Države, 21.04.2018. - 25.04.2018

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
BPC 157 ; abdominal aorta occlusion

Sažetak
Aim We focused abdominal aorta occlusion in rats and the therapy with the stable gastric pentadecapeptide BPC 157. Effective in perforated stomach lesion and perforated caecum lesion as well as ischemic colitis, ischemia and reperfusion, BPC 157 rapidly activates vessels ”running“ toward the perforated defect (stomach perforation (Gastroenterology 2017 ; 152, Issue 5, Supplement 1, S889–S890 ; Gastroenterology 2017 ; 152, Issue 5, Supplement 1, S890–S891) or to bypass vessel obstruction (ischemic colitis) (World Journal of Gastroenterology, in press, 2017) and largely attenuates lesions course. In this study, we investigated the effect of pentadecapeptide BPC 157 on rapid activation of collateral circulation after acute occlusion of the abdominal aorta in rats. Methods Female Wistar Albino rats, 300 ± 20 g b.w. were used. Animals were randomized to control and pentadecapeptide BPC 157 treated group. Deeply anesthetised (diazepam 6 mg/kg i.p., thiopental 40 mg/kg i.p.) rats underwent abdominal aorta ligation with surgical suture at the level between a right iliolumbar artery and the bifurcation of the aorta. Medication was 1 ml bath, either BPC 157 10 μg/kg or saline, given immediately after abdominal aorta ligation at the site of the ligation. The digital subtraction angiography (DSA) assessment was at the 1 h ligation time. A fluoroscopy unit (Shimadzu type C-Vision Plus, Tokyo, Japan) and a nonionic contrast medium (Iohexol ; Omnipaque 350, GE Healthcare, Chicago, USA) given as 2 ml, 0.3 ml/sec in abdominal aorta closely above ligation site. Collateralization (presentation of superior and inferior mesenteric arteries and their interconnection (arch of Riolan), right and left ovarian arteries, lumbar arteries) was estimated at end of arterial DSA phase just before venous phase initiation, analyzed using the ISSA (VAMSTEC- Software Company, Zagreb, Croatia) image program. Results Controls. Regularly rats underwent abdominal aorta occlusion between a right iliolumbar artery and the bifurcation of the aorta exhibit abdominal aorta failure with occlusion presenting with the very poor presentation of the collaterals, exhibiting only limited presentation of the superior mesenteric artery and arch of Riolan. BPC 157. We noted an immediate presentation of superior and inferior mesenteric arteries and their interconnection (arch of Riolan), right and left ovarian arteries, lumbar arteries in all rats underwent abdominal aorta occlusion that received BPC 157 therapy (Fig. 1). Conclusion Stable gastric pentadecapeptide BPC 157 may be the therapy of abdominal aorta occlusion in rats.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

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