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Pregled bibliografske jedinice broj: 1260297

Engineering recombinant Lactococcus lactis as a delivery vehicle for BPC-157 peptide with antioxidant activities

Škrlec, Katja; Ručman, Rudolf; Jarc, Eva; Sikirić, Predrag; Švajger, Urban; Petan, Toni; Perišić Nanut, Milica; Štrukelj, Borut; Berlec, Aleš
Engineering recombinant Lactococcus lactis as a delivery vehicle for BPC-157 peptide with antioxidant activities // Applied Microbiology and Biotechnology, 102 (2018), 23; 10103-10117 doi:10.1007/s00253-018-9333-6 (međunarodna recenzija, članak, znanstveni)

CROSBI ID: 1260297 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Engineering recombinant Lactococcus lactis as a delivery vehicle for BPC-157 peptide with antioxidant activities

Autori
Škrlec, Katja ; Ručman, Rudolf ; Jarc, Eva ; Sikirić, Predrag ; Švajger, Urban ; Petan, Toni ; Perišić Nanut, Milica ; Štrukelj, Borut ; Berlec, Aleš

Izvornik
Applied Microbiology and Biotechnology (0175-7598) 102 (2018), 23; 10103-10117

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Antioxidant ; BPC-157 ; Fibroblasts ; Lactococcus lactis ; Peptide delivery ; Recombinant

Sažetak
Lactic acid bacteria (LAB) are attractive hosts for the expression of heterologous proteins and can be engineered to deliver therapeutic proteins or peptides to mucosal surfaces. The gastric stable pentadecapeptide BPC-157 is able to prevent and treat gastrointestinal inflammation by reducing the production of reactive oxygen species (ROS). In this study, we used LAB Lactococcus lactis as a vector to deliver BPC-157 by surface display and trypsin shedding or by secretion to the growth medium. Surface display of BPC-157 was achieved by fusing it with basic membrane protein A (BmpA) or with the peptidoglycan binding domain of AcmA and Usp45 secretion signal. While the expression of BmpA-fusion proteins was higher than that of AcmA/Usp45-fusion protein, the surface display ability of BPC-157 was approximately 14- fold higher with AcmA/Usp45-fusion protein. Release of BPC-157 from the bacterial surface or from isolated fusion proteins by trypsinization was demonstrated with anti-BPC-157 antibodies or by mass spectrometry. The concentration of BPC-157 delivered by surface display via AcmA/Usp45-fusion was 30 ng/ml. This increased to 117 ng/ml by Usp45 signal-mediated secretion, making the latter the most effective lactococcal delivery approach for BPC-157. Secreted BPC-157 significantly decreased ROS production in 149BR fibroblast cell model, suggesting its potential benefit in the treatment of intestinal inflammations. Additionally, a comparison of different modes of small peptide delivery by L. lactis, performed in the present study, will facilitate the future use of L. lactis as peptide delivery vehicle.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti



POVEZANOST RADA

Projekti:
MZOS-108-1083570-3635 - Pentadekapeptid BPC 157 - daljnja istraživanja (Sikirić, Predrag, MZOS ) ( CroRIS)

Ustanove:
Medicinski fakultet, Zagreb

Profili:

Avatar Url Predrag Sikirić (autor)

Poveznice na cjeloviti tekst rada:

doi

Citiraj ovu publikaciju:

Škrlec, Katja; Ručman, Rudolf; Jarc, Eva; Sikirić, Predrag; Švajger, Urban; Petan, Toni; Perišić Nanut, Milica; Štrukelj, Borut; Berlec, Aleš
Engineering recombinant Lactococcus lactis as a delivery vehicle for BPC-157 peptide with antioxidant activities // Applied Microbiology and Biotechnology, 102 (2018), 23; 10103-10117 doi:10.1007/s00253-018-9333-6 (međunarodna recenzija, članak, znanstveni)
Škrlec, K., Ručman, R., Jarc, E., Sikirić, P., Švajger, U., Petan, T., Perišić Nanut, M., Štrukelj, B. & Berlec, A. (2018) Engineering recombinant Lactococcus lactis as a delivery vehicle for BPC-157 peptide with antioxidant activities. Applied Microbiology and Biotechnology, 102 (23), 10103-10117 doi:10.1007/s00253-018-9333-6.
@article{article, author = {\v{S}krlec, Katja and Ru\v{c}man, Rudolf and Jarc, Eva and Sikiri\'{c}, Predrag and \v{S}vajger, Urban and Petan, Toni and Peri\v{s}i\'{c} Nanut, Milica and \v{S}trukelj, Borut and Berlec, Ale\v{s}}, year = {2018}, pages = {10103-10117}, DOI = {10.1007/s00253-018-9333-6}, keywords = {Antioxidant, BPC-157, Fibroblasts, Lactococcus lactis, Peptide delivery, Recombinant}, journal = {Applied Microbiology and Biotechnology}, doi = {10.1007/s00253-018-9333-6}, volume = {102}, number = {23}, issn = {0175-7598}, title = {Engineering recombinant Lactococcus lactis as a delivery vehicle for BPC-157 peptide with antioxidant activities}, keyword = {Antioxidant, BPC-157, Fibroblasts, Lactococcus lactis, Peptide delivery, Recombinant} }
@article{article, author = {\v{S}krlec, Katja and Ru\v{c}man, Rudolf and Jarc, Eva and Sikiri\'{c}, Predrag and \v{S}vajger, Urban and Petan, Toni and Peri\v{s}i\'{c} Nanut, Milica and \v{S}trukelj, Borut and Berlec, Ale\v{s}}, year = {2018}, pages = {10103-10117}, DOI = {10.1007/s00253-018-9333-6}, keywords = {Antioxidant, BPC-157, Fibroblasts, Lactococcus lactis, Peptide delivery, Recombinant}, journal = {Applied Microbiology and Biotechnology}, doi = {10.1007/s00253-018-9333-6}, volume = {102}, number = {23}, issn = {0175-7598}, title = {Engineering recombinant Lactococcus lactis as a delivery vehicle for BPC-157 peptide with antioxidant activities}, keyword = {Antioxidant, BPC-157, Fibroblasts, Lactococcus lactis, Peptide delivery, Recombinant} }

Časopis indeksira:


  • Current Contents Connect (CCC)
  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE

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