Naslov
GMO analysis in Croatia: current approaches and new challenges

Autori
Hanzer, Renata

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Abstracts of the Food Safety and Quality Congress with international participation "New Achievements and Future Challenges" / Šostar, Zvonimir - Zagreb : Institut za medicinska istraživanja i medicinu rada, 2017, 31-31

Skup
Food Safety and Quality Congress with international participation “New Achievements and Future Challenges”

Mjesto i datum
Opatija, Hrvatska, 21.11.2017. - 24.11.2017

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
EU legislation ; laboratories ; monitoring ; PCR

Sažetak
Since the beginning of commercialisation of genetically modified plants in 1994, we have been dealing with the challenges of control and monitoring through policies and laboratory analysis. An increasing number of genetically modified crops, along with the development of new types of genetic modifications, pose a challenge for enforcement laboratories. Reliable analysis is needed to ensure the implementation of national and EU regulation. In Croatia, as well as in the European Union, real-time polymerase chain reaction (real- time PCR) is still the method of choice for detection, identification, and quantification of genetically modified organisms in authorised laboratories. The method is applicable to a large number of matrices, from raw materials to highly processed samples. It provides high level of specificity and sensitivity but as the number of authorised GM events and their complexity have increased, the method has become more and more timeconsuming and, consequently, expensive. Use of a primer pair and a probe, which amplifies a specific target, makes this method suitable for all authorised GM events, but the presence of unauthorised evets can only be insinuated. The necessity to face the challenges has led to the development of new detection approaches and new methods such as multiple targeting PCR, digital PCR, next generation sequencing, and others. Although the current approach meets our legislation requirements, it is expected that implementation of new methodologies will be needed in the near future.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Poljoprivreda (agronomija), Biotehnologija, Prehrambena tehnologija

POVEZANOST RADA