Naslov
Cyclodextrin-based displacement strategy of sterigmatocystin from serum albumin as a novel approach for acute poisoning detoxification

Autori
Jakšić, Daniela ; Šegvić Klarić, Maja ; Rimac, Hrvoje ; Kerep, Robert ; Piantanida, Ivo

Izvornik
International journal of molecular sciences (1422-0067) 24 (2023); 4485, 15

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
mycotoxin sterigmatocystin ; cyclodextrin ; competitive binding ; fluorescence ; circular dichroism

Sažetak
This study demonstrates that sterigmatocystin (STC) interacts non-covalently with various cyclodextrins (CDs), showing the highest binding affinity for sugammadex (a gama-CD derivative) and gama-CD, and an almost order of magnitude lower affinity for beta-CD. This difference in affinity was studied using molecular modelling and fluorescence spectroscopy, which demonstrated a better insertion of STC into larger CDs. In parallel, we showed that STC binds to human serum albumin (HSA) (a blood protein known for its role as a transporter of small molecules) with an almost two order of magnitude lower affinity compared to sugammadex and gama-CD. Competitive fluorescence experiments clearly demonstrated an efficient displacement of STC from the STC–HSA complex by cyclodextrins. These results are a proof-of-concept that CDs can be used to complex STC and related mycotoxins. Similarly, as sugammadex extracts neuromuscular relaxants (e.g., rocuronium and vecuronium) from blood and blocks their bioactivity, it could also be used as first aid upon acute intoxication to encapsulate a larger part of the STC mycotoxin from serum albumin.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

POVEZANOST RADA