Naslov
Development of Novel Method for Determination of Disinfection Agent N-(3- aminopropyl)-N-dodecylpropane-1,3-diamine Using HPLC-DAD

Autori
Tomazin, Lovro ; Čižmek, Lara ; Mazur, Dmitrii ; Trebše, Polonca

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
22nd European Meeting on Environmental Chemistry : Book of Abstracts / Bavcon Kralj, Mojca ; Trebše, Polonca ; Prosenc, Franja ; Šunta, Urška ; Čižmek, Lara - Ljubljana : Univerza v Ljubljani, 2022, 135-135

ISBN
978-961-297-034-5

Skup
22nd European Meeting on Environmental Chemistry (EMEC22)

Mjesto i datum
Ljubljana, Slovenija, 05.12.2022. - 08.12.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Disinfectant ; Diamine ; Chromatography

Sažetak
Disinfectants are one of the key tools in the process of controlling the spread of hospital infections. Given that there is a growing interest in the development of more environmentally and human-friendly, but also economically affordable agents, the reduction of active substances in prepared working solutions is desirable. At the same time, the prepared solutions must keep or even shorten needed contact times, which are necessary to achieve the required effects according to standardized efficacy testing procedures. Quaternary ammonium compounds and amines represent one of the most common active groups of substances used in preparations to disinfect surfaces, instruments, equipment, or remaining medical devices. The reason for their widespread use is that these substances enable different ways of application, good compatibility with materials, and generally proven effectiveness. The problem of monitoring such a large group of compounds in so many different media is needed in an extremely large number of analytical methods, which include chromatography (liquid or gas coupled with various detectors), mass spectrometry, electrochemical, and many other methods. A reliable analytical method is also vital in process of proving compliance with legislation in the field of biocides, especially in the field of medical devices. Antimicrobial substance N-(3-aminopropyl)-N-dodecylpropane-1, 3-diamine (BAPDA) is a novel compound used for disinfection purposes. Methodologies for BAPDA detection such as HPLC-MS have already been developed, but our scope of research was oriented to the development of a simple method, suitable for the determination of residues on various hygienic and medical surfaces. We propose HPLC-DAD method with the prior derivatization of the amine, present in the sample. The derivatization procedure was carried out as described earlier [4]. Briefly, amine was dissolved in a 0.1 M HCl aqueous solution or ethyl acetate. Sodium hydroxide (2 M) was added followed by the addition of benzoyl chloride. The reaction took place at different temperatures for a specific time interval. The acylation was stopped by adding saturated NaCl solution and performing further extraction with diethyl ether. The upper organic layer was dried and redissolved in methanol for HPLC analysis. Method optimization included: the selection of solvent for a working solution, temperature, and derivatization time optimization. Obtained results showed that ethyl acetate was a better initial solvent, with 50 min as an optimal time at 30°C with a linear response curve for amine concentrations from 0.2 to 1 mg/ml. The method was evaluated on several biocidal products.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Kemijsko inženjerstvo

POVEZANOST RADA