Naslov
EFFECTS OF LOW DOSE TETANUS TOXIN INJECTIONS INTO THE RAT MOTOR CORTEX AND BASAL GANGLIA ON MOTOR PERFORMANCE

Autori
Meglić, Patrik ; Šoštarić, Petra ; Virag, Davor ; Matak, Ivica

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Pharmacia 2022 / Mršić Pelčić, Jasenka ; Vitezić, Dinko ; Janković, Tamara - Zagreb, 2022, 178-178

Skup
10. hrvatski kongres farmakologije ; 1. hrvatski kongres kliničke farmakologije s međunarodnim sudjelovanjem

Mjesto i datum
Opatija, Hrvatska, 22.09.2022. - 25.09.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
basal ganglia, tetanus toxin, movement disorders

Sažetak
Introduction: Abnormalities in the motor cortex (Mc) and basal ganglia excitability play a central role in movement disorders such as dystonias and parkinsonism. Tetanus neurotoxin (TeNT) prevents the inhibitory neurotransmission in central synapses. The aim of the experiments was to examine the behavioral effect of neuronal disinhibition in mentioned brain regions induced by low, non-convulsive doses of TeNT. Materials and methods: In the first experiment rats were unilaterally injected into the caudate putamen (CPu) or Mc with 0.5 ng TeNT. The injections were repeated into the contralateral motor regions after 2 weeks, and the effect of TeNT was assessed for another 2 weeks. In the another preliminary experiment, rats were unulateraly injected with 0.5 ng of TeNT into the globus pallidus internus (GPi) and substantia nigra (SN), or SN alone. Different behavioral tests were performed repeatedly to assess the effect of TeNT induced disinhibition on motor performance, as well as to exclude possible epileptogenic action of TeNT. Results: The Mc or CPu disinhibition with TeNT induces subtle motor impairment during the narrow beam crossing in 50% of treated animals, without signs of epileptogenic action. Animals injected into the GPi and SN, or SN alone, showed ipsilateral circling tendency in open field (50%) and swimming tests (100%). Conclusion: TeNT injected into defined motor regions can induce motor impairments of different modalities, depending on the site of injection. While subtle motor impairment after CPu and MC disinhibition require aggravation by contralateral regional disinhibition, unilateral GPi od SN disinhibition induced more prominent motor impairment. Funding: Croatian Science Foundation (project ID: UIP-2019-04-8277)

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

POVEZANOST RADA