Naslov
Low Dose Tetanus Toxin Injections into the Rat Motor Cortex and Striatum Impair the Narrow Beam Walking Performance

Autori
Meglić, Patrik ; Šoštarić, Petra ; Matak, Ivica

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
The 17th International Symposium on Cholinergic Mechanisms Programme and Abstracts / Kovarik Z., Primožič I. - Zagreb : Institut za medicinska istraživanja i medicinu rada, 2022, 67-67

ISBN
978-953-96817-8-2

Skup
17th International Symposium on Cholinergic Mechanisms, ISCM2022

Mjesto i datum
Dubrovnik, Hrvatska, 08.05.2022. - 12.05.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
basal ganglia, tetanus toxin, movement disorders

Sažetak
Basal ganglia and motor cortex integrate the sensory proprioceptive input arriving from periphery and the planning and execution of movement. Abnormalities in the motor cortex and basal ganglia excitability play a central role in the onset of movement disorders such as dystonias and parkinsonism. Tetanus neurotoxin (TeNT) prevents the inhibitory neurotransmission in central synapses. The aim of present study was to examine the behavioral effect of neuronal disinhibition in mentioned brain regions induced by low, non-convulsive doses of TeNT. The rats were injected unilaterally into caudate putamen or motor cortex with 0.5 ng TeNT. After 2 weeks, the injections were repeated into the aforementioned contralateral motor regions, and the effect of TeNT was assessed for another 2 weeks. Various behavioral tests were repeatedly performed to assess the effect of TeNT-induced disinhibition on normal motor performance in rats, as well as to exclude possible epileptogenic toxin action. First unilateral toxin injection exerted a short-lasting impairment in the ability to transverse a narrow beam, and with no visible effects on rota-rod, swimming, gait analysis (catwalk) and EMG. Upon the second toxin injection, the animals developed a lasting impairment in the beam walking performance while the performance in other motor tests remained unimpaired. The plantar misplacement of the hind-limb during the narrow beam traversing were more evident on the hind-paw contralateral to the injected brain region. Open field test, pre-pulse inhibition and attempted audiogenic seizure tests did not indicate the possible epileptogenic actions of TeNT. The motor cortex or striatal disinhibition with TeNT induces subtle motor impairment in the relatively complex motor task of crossing the elevated narrow beam, requiring correct prediction of paw placement. After unilateral toxin applications, the animals appear to be able to compensate the proprioceptive motor deficit, which is then aggravated and becomes long lasting after contralateral regional disinhibition. Funding: Croatian Science Foundation (project ID: UIP-2019-04-8277)

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

POVEZANOST RADA