Naslov
Acute galactose remodels energy metabolism in rats hypothalamus after oral administration

Autori
Knezović, Ana ; Vidović, Ante ; Zima, David ; Homolak, Jan ; Babić Perhoč, Ana ; Osmanović Barilar, Jelena ; Salkovćc-Petrišić, Melita

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, neobjavljeni rad, znanstveni

Skup
15th​ International Conference on Alzheimer’s and Parkinson’s Diseases and related neurological disorders, AD/PD™ 2021

Mjesto i datum
Virtual conference, 09.03.2021. - 14.03.2021

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
galactose ; metabolism ; hypothalamus

Sažetak
Objectives In our previous research, we showed that long-term oral galactose improves cognitive deficit in rat model of sporadic Alzheimer's disease. We aimed to explore the effect of single acute oral galactose administration on peripheral oxidative stress and hypothalamic metabolism in healthy rats. Methods Three-month old male Wistar rats were treated orally with galactose (200mg/kg) and sacrificed 30, 60 or 120 minutes after. Glucose and galactose concentration, superoxide dismutase activity, low molecular weight thiols and lipid peroxidation were measured in plasma. Hypothalamic pyruvate dehydrogenase (PHD), cytochrome c oxidase (COXIV), cytochrome c (CytC) and caspase-3 (CASP3) levels were measured by Western blot. Results With the exception of reduced lipid peroxidation end products 30 and 120 min after oral galactose treatment in comparison to controls, none of the other measured parameters in plasma were affected by galactose treatment. In hypothalamus both PHD and CASP3 expression was found decreased after oral galactose administration, while COXIV expression was found increased 60 and 120 minutes after oral galactose. There was no change in hypothalamic CytC expression. Conclusions Acute oral galactose decreases peripheral lipid peroxidation and changes metabolic pattern in hypothalamus by promoting increased COXIV expression and preventing cell apoptosis that can have a substantial role in brain function.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA