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Pregled bibliografske jedinice broj: 1253161

Mitochondrial Dysfunction in a High Intraocular Pressure-Induced Retinal Ischemia Minipig Model

Pasák, Michael; Vanišová, Marie; Tichotová, Lucie; Křížová, Jana; Ardan, Taras; Nemesh, Yaroslav; Čížková, Jana; Kolesnikova, Anastasiia; Nyshchuk, Ruslan; Josifovska, Natasha et al.
Mitochondrial Dysfunction in a High Intraocular Pressure-Induced Retinal Ischemia Minipig Model // Biomolecules, 12 (2022), 10; 1532, 13 doi:10.3390/biom12101532 (međunarodna recenzija, članak, znanstveni)

CROSBI ID: 1253161 Za ispravke kontaktirajte CROSBI podršku putem web obrasca

Naslov
Mitochondrial Dysfunction in a High Intraocular Pressure-Induced Retinal Ischemia Minipig Model

Autori
Pasák, Michael ; Vanišová, Marie ; Tichotová, Lucie ; Křížová, Jana ; Ardan, Taras ; Nemesh, Yaroslav ; Čížková, Jana ; Kolesnikova, Anastasiia ; Nyshchuk, Ruslan ; Josifovska, Natasha ; Lytvynchuk, Lyubomyr ; Kolko, Miriam ; Motlík, Jan ; Petrovski, Goran ; Hansíková, Hana

Izvornik
Biomolecules (2218-273X) 12 (2022), 10; 1532, 13

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
minipig ; retinal ischemia ; mitochondrial dysfunction ; coenzyme Q10

Sažetak
Purpose: Retinal ischemia (RI) and progressive neuronal death are sight-threatening conditions. Mitochondrial (mt) dysfunction and fusion/fission processes have been suggested to play a role in the pathophysiology of RI. This study focuses on changes in the mt parameters of the neuroretina, retinal pigment epithelium (RPE) and choroid in a porcine high intraocular pressure (IOP)-induced RI minipig model. Methods: In one eye, an acute IOP elevation was induced in minipigs and compared to the other control eye. Activity and amount of respiratory chain complexes (RCC) were analyzed by spectrophotometry and Western blot, respectively. The coenzyme Q10 (CoQ10) content was measured using HPLC, and the ultrastructure of the mt was studied via transmission electron microscopy. The expression of selected mt-pathway genes was determined by RT-PCR. Results: At a functional level, increased RCC I activity and decreased total CoQ10 content were found in RPE cells. At a protein level, CORE2, a subunit of RCC III, and DRP1, was significantly decreased in the neuroretina. Drp1 and Opa1, protein-encoding genes responsible for mt quality control, were decreased in most of the samples from the RPE and neuroretina. Conclusions: The eyes of the minipig can be considered a potential RI model to study mt dysfunction in this disease. Strategies targeting mt protection may provide a promising way to delay the acute damage and onset of RI.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Temeljne medicinske znanosti



POVEZANOST RADA

Ustanove:
KBC Split,
Medicinski fakultet, Split

Poveznice na cjeloviti tekst rada:

doi

Citiraj ovu publikaciju:

Pasák, Michael; Vanišová, Marie; Tichotová, Lucie; Křížová, Jana; Ardan, Taras; Nemesh, Yaroslav; Čížková, Jana; Kolesnikova, Anastasiia; Nyshchuk, Ruslan; Josifovska, Natasha et al.
Mitochondrial Dysfunction in a High Intraocular Pressure-Induced Retinal Ischemia Minipig Model // Biomolecules, 12 (2022), 10; 1532, 13 doi:10.3390/biom12101532 (međunarodna recenzija, članak, znanstveni)
Pasák, M., Vanišová, M., Tichotová, L., Křížová, J., Ardan, T., Nemesh, Y., Čížková, J., Kolesnikova, A., Nyshchuk, R. & Josifovska, N. (2022) Mitochondrial Dysfunction in a High Intraocular Pressure-Induced Retinal Ischemia Minipig Model. Biomolecules, 12 (10), 1532, 13 doi:10.3390/biom12101532.
@article{article, author = {Pas\'{a}k, Michael and Vani\v{s}ov\'{a}, Marie and Tichotov\'{a}, Lucie and K\v{r}\'{\i}\v{z}ov\'{a}, Jana and Ardan, Taras and Nemesh, Yaroslav and \v{C}\'{\i}\v{z}kov\'{a}, Jana and Kolesnikova, Anastasiia and Nyshchuk, Ruslan and Josifovska, Natasha and Lytvynchuk, Lyubomyr and Kolko, Miriam and Motl\'{\i}k, Jan and Petrovski, Goran and Hans\'{\i}kov\'{a}, Hana}, year = {2022}, pages = {13}, DOI = {10.3390/biom12101532}, chapter = {1532}, keywords = {minipig, retinal ischemia, mitochondrial dysfunction, coenzyme Q10}, journal = {Biomolecules}, doi = {10.3390/biom12101532}, volume = {12}, number = {10}, issn = {2218-273X}, title = {Mitochondrial Dysfunction in a High Intraocular Pressure-Induced Retinal Ischemia Minipig Model}, keyword = {minipig, retinal ischemia, mitochondrial dysfunction, coenzyme Q10}, chapternumber = {1532} }
@article{article, author = {Pas\'{a}k, Michael and Vani\v{s}ov\'{a}, Marie and Tichotov\'{a}, Lucie and K\v{r}\'{\i}\v{z}ov\'{a}, Jana and Ardan, Taras and Nemesh, Yaroslav and \v{C}\'{\i}\v{z}kov\'{a}, Jana and Kolesnikova, Anastasiia and Nyshchuk, Ruslan and Josifovska, Natasha and Lytvynchuk, Lyubomyr and Kolko, Miriam and Motl\'{\i}k, Jan and Petrovski, Goran and Hans\'{\i}kov\'{a}, Hana}, year = {2022}, pages = {13}, DOI = {10.3390/biom12101532}, chapter = {1532}, keywords = {minipig, retinal ischemia, mitochondrial dysfunction, coenzyme Q10}, journal = {Biomolecules}, doi = {10.3390/biom12101532}, volume = {12}, number = {10}, issn = {2218-273X}, title = {Mitochondrial Dysfunction in a High Intraocular Pressure-Induced Retinal Ischemia Minipig Model}, keyword = {minipig, retinal ischemia, mitochondrial dysfunction, coenzyme Q10}, chapternumber = {1532} }

Časopis indeksira:


  • Web of Science Core Collection (WoSCC)
    • Science Citation Index Expanded (SCI-EXP)
    • SCI-EXP, SSCI i/ili A&HCI
  • Scopus
  • MEDLINE

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