Naslov
Chemical genetics screen identifies COPB2 tool compounds that alters ER stress response and induces RTK dysregulation in lung cancer cells

Autori
Saraon, Punit ; Snider, Jamie ; Schormann, Wiebke ; Rai, Ankit ; Radulovich, Nikolina ; Sánchez-Osuna, Maria ; Coulombe-Huntington, Jasmin ; Huard, Caroline ; Mohammed, Mohammed ; Lima-Fernandes, Evelyne ; Thériault, Brigitte ; Halabelian, Levon ; Chan, Manuel ; Joshi, Dhananjay ; Drecun, Luka ; Yao, Zhong ; Pathmanathan, Shivanthy ; Wong, Victoria ; Lyakisheva, Anna ; Aboualizadeh, Farzaneh ; Niu, Li ; Li, Fengling ; Kiyota, Taira ; Subramanian, Ratheesh ; Joseph, Babu ; Aman, Ahmed ; Prakesch, Michael ; Isaac, Methvin ; Mamai, Ahmed ; Poda, Gennady ; Vedadi, Masoud ; Marcellus, Richard ; Uehling, David ; Leighl, Natasha ; Sacher, Adrian ; Samaržija, Miroslav ; Jakopović, Marko ; Arrowsmith, Cheryl ; Tyers, Mike ; Tsao, Ming-Sound ; Andrews, David ; Al-awar, Rima ; Štagljar, Igor

Izvornik
Journal of molecular biology (0022-2836) 433 (2021), 23; 167294, 19

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
coatomer protein complex beta 2 (COPB2) ; epidermal growth factor receptor (EGFR) ; lung cancer ; endoplasmic reticulum stress ; receptor tyrosine kinase

Sažetak
Activating mutations in the epidermal growth factor receptor (EGFR) are common driver mutations in nonsmall cell lung cancer (NSCLC). First, second and third generation EGFR tyrosine kinase inhibitors (TKIs) are effective at inhibiting mutant EGFR NSCLC, however, acquired resistance is a major issue, leading to disease relapse. Here, we characterize a small molecule, EM166, an analog of a small molecule which we previously identified to inhibit mutant EGFR signalling via a novel mechanism of action. We show that EM166 attenuates receptor tyrosine kinase (RTK) expression and signalling and alters the electrophoretic mobility of Coatomer Protein Complex Beta 2 (COPB2) protein in mutant EGFR NSCLC cells. Moreover, we demonstrate that EM166 can alter the subcellular localization of EGFR and COPB2 within the early secretory pathway. Furthermore, we find that COPB2 knockdown reduces the growth of mutant EGFR lung cancer cells, alters the post-translational processing of RTKs, and alters the endoplasmic reticulum (ER) stress response pathway. Lastly, we show that EM166 treatment also alters the ER stress response pathway and inhibits the growth of mutant EGFR lung cancer cells and organoids. Our results demonstrate that targeting of COPB2 with EM166 presents a viable approach to attenuate mutant EGFR signalling and growth in NSCLC.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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