Naslov
Optimizing the method omitting cell lysis and protein extraction for identification of bacteria from positive blood cultures by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry
(Optimizing the method omitting cell lysis and protein extraction for identification of bacteria from positive blood cultures by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry)

Autori
Perše, Gabrijela ; Bošnjak, Zrinka ; Mareković, Ivana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, neobjavljeni rad, znanstveni

Skup
32nd ECCMID

Mjesto i datum
Lisabon, Portugal, 23.04.2022. - 26.04.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
MALDI-TOF mass spectrometry ; blood culture ; sepsis

Sažetak
In comparison to similar protocols omitting cell lysis and protein extraction, we demonstrated a similar performance for the identification of gram-negative and superior performance for the identification of Gram-positive bacteria from positive blood cultures. This could be explained by including both the additional spin step and wash step in the procedure. Although in-house protocols aare fast, easy and inexpensive, extraction with methods such as Sepsityper is the solution if we want to provide a greater rate of precision for the identification of gram-poitive bacteria.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

POVEZANOST RADA