Naslov
Optimization of grape marc polyphenols extraction assisted by simultaneous ultrasound and microwave irradiation

Autori
Gunjević, Veronika ; Boffa, Luisa ; Cravotto, Giancarlo

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
GENP 2018 III Edition “Green Extraction of Natural Products”: Book of abstracts / Lisa Clodoveo, Maria ; Corbo, Filomena ; Amirante, Riccardo - Bari : Universita degli studi di Bari "Aldo Moro", 2018, 86-86

ISBN
978-88-6629-011-7

Skup
Green Extraction of Natural Products (GENP2018)

Mjesto i datum
Bari, Italija, 12.11.2018. - 13.11.2018

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Simultaneous ultrasound/microwaves ; natural deep eutectic solvents ; polyphenols ; optimization

Sažetak
Objectives Polyphenols from wine industry by-products and wastes may be active components of interest for food, cosmetics and pharmaceutical production. The recovery of these compounds is commonly performed by solvent-extraction procedures with conventional techniques, that may show drawbacks in term of environmental impact. Aiming to design a green extraction procedure for grape marc polyphenols, simultaneous ultrasound and microwave (US/MW) assisted extractions were optimized by response surface methodology (RSM). As a green alternative to conventional solvents, a natural deep eutectic solvent (NADES) was used. Methods Based on the preliminary experiments, the NADES choline chloride:citric acid (Ch:Cit) with 30 % of water (w/w) was selected. The extractions were performed under simultaneous US/MW irradiation. The parameters studied were: time of extraction (5 to 15 min), MW power (200 to 400 W) and temperature (50 to 90 °C). Total polyphenolic content (TPC) was determined using Folin-Ciocalteu assay and HPLC analysis. The optimization of aforementioned parameters was carried out by RSM using Design Expert 7.0.0 software based on experimental data derived by implementing Box- Behnken experimental plan. Detailed statistical analysis of the obtained model, analysis of variance (ANOVA), was also provided using Design Expert 7.0.0. Results Optimal extraction conditions for achieving the maximum TPC are: time 10 min, MW power 300 W and temperature 80 °C. By applying the listed conditions, TPC yield was 59.53 mgGAE gDW-1. RSM using experimental data, could provide the extraction model. By ANOVA statistical analysis, it has been proven that the approximation of the experimental TPC yields with the modelled TPC yields is statistically significant, which means that the model application is justified for the investigated area. The two variables with the greatest impact on the TPC yield are extraction time and temperature. This study presents a fast and environmentally friendly extraction process of valuable biologically active compounds from grape marc, paving the way for further industrial development.

Izvorni jezik
Engleski

Znanstvena područja
Kemija

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