Naslov
THE INFLUENCE OF TUBE SEPARATOR GEL ON MEASUREMENT OF VALPROIC ACID IN SERUM

Autori
Cesar Kocijan, Valentina ; Đuras, Anamarija ; Ostroški, Ivanka ; Rade, Anamarija ; Kelava, Željka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Clin Chem Lab Med 2021 ; 59, Special Suppl / - : Clinical Chemistry and Laboratory Medicine, 2021, W259-W259

Skup
EuroMedLab Munich 2021

Mjesto i datum
München, Njemačka, 10.04.2022. - 14.04.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
valproati ; separator gel
(valproic acid ; separator gel)

Sažetak
BACKGROUND-AIM: Therapeutic drug monitoring of antiepileptic drugs is being largely used to optimize treatment and to assessnonadherence.The effect of the gel separator in blood sampling tubes on the concentration of drugs has been shown by many studies. The aim of the study was to determine whether the separator gel in serum tubes influences to the concentration of valproic acid at baseline measurement, after 4h and 24h. METHODS: Blood samples were collected from 20 volunteers in 5 mL SST II Advance tube (SST) and clot activator tube (CAT)(Beckton, Dickinson and Company, Franklin Lakes, USA) and centrifuged at 3040 g for 10 minutes. For each vacutainer, valproic acid concentration was determined at baseline, after 4h and 24h on the Abbott SR 1000 analyzer (Abbott Laboratories, Illinois, USA) by the chemiluminescent microparticle immunoassay (CMIA). Between analyses, samples were capped and stored at 4-8°C. Statistical analysis was performed with MedCalc Statistical Software version 14.3.0(MedCalc Software, Belgium). The distribution of normality was tested with the Kolmogorov-Smirnov test and data are presented as average (X) and standard deviation(SD) for each tube type. The comparison was assessed by repeated measures ANOVA. P value <0.05 was considered statistically significant. The criteria for clinical significance was the significant change limit (SLC) calculated as reference value ± 2.8xSD of the internal quality control. The mean value of valproic acid concentration at baseline in CAT tube served as a reference value and SD of the internal quality control was 1.23, yielding the acceptable range of 51.3±3.47. RESULTS: The following valproic acid concentrations were obtained: at baseline 51.3(±18.9) for CAT tube and 51.2 (±18.0) for SST tube ; after 4h 50.9(±18.9) for CAT tube and 50.6(±16.5) for SST tube ; after 24h 50.6(±19.9) for CAT tube and 51.9(±19.53)for SST tube. There is no statistically significant difference between both tube at baseline measurement (P=0.93) and after 4h(P=0.59), but there was statistically significant difference after 24h (P=0.04). The arithmetic mean in SST tube after 24h was within the clinical range based on SLC. CONCLUSIONS: These results showed that both types of vacutainer are suitable for measuring valproic acid, and concentrations were stable up to 24h when samples were stored at 4-8°C.

Izvorni jezik
Engleski

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