Naslov
Supercritical CO2 extraction of phenolic compounds from medicinal plants

Autori
Čižmek, Lara ; Bavcon Kralj, Mojca ; Čož-Rakovac, Rozelindra ; Mazur, Dmitrii ; Ul’yanovskii, Nikolay ; Likon, Marko ; Trebše, Polonca

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Chem2Change, Environmental Chemistry towards Global Change, 2nd Online ACE Seminar on Chemistry and the Environment Led by Early-Career Scientists : Book of Abstracts / Niemi, Lydia ; Pap, Szabolcs ; Prosenc, Franja ; Šunta, Urška ; Mišíková, Frederika ; Krejčová, Anna ; Tykva, Marek ; Gajica, Gordana ; Savić, Slađana - Pardubice : University of Pardubice, 2022, 24-24

ISBN
978-80-7560-406-4

Skup
2nd Online ACE Seminar on Chemistry and the Environment Environmental Chemistry towards Global Change (Chem2Change 2022)

Mjesto i datum
Online, 15.03.2022. - 16.03.2022

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Nije recenziran

Ključne riječi
plants ; square-wave voltammetry ; liquid chromatography ; mass spectrometry
(plants ; square-wave voltammetry ; liquid chromatography ; gas chromatography ; mass spectrometry)

Sažetak
The usage of environmentally friendly extraction techniques for the discovery of novel synthetic compounds available in medicinal plants has a high priority with everyday advances in the field of pharmaceuticals. In this research, a supercritical CO2 extraction method was implemented in the analysis of four plants (chamomile, St. John’s wort, yarrow, and curry plant) after which the non-targeted analysis of the chemical composition, phenolic content, and antioxidant activity was evaluated. The obtained extraction yield was the highest for the chamomile (5%) sample. The chemical composition analyzed by gas chromatography-high-resolution mass spectrometry (GC-HRMS) and liquid chromatography-high-resolution mass spectrometry (LC-HRMS) demonstrated extraction of diverse compounds including terpenes and terpenoids, fatty acids, flavonoids and coumarins, functionalized phytosterols, and polyphenols. Voltammetry of microfilm immobilized on a glassy carbon electrode using square-wave voltammetry (SWV) was applied in the analysis of extracts. It was found that antioxidant activity obtained by SWV correlates well to 1, 1-diphenyl-2-picrylhidrazine (DPPH) radical assay (R2=0.818) and ferric reducing antioxidant power (FRAP) assay (R2=0.640), but not to the total phenolic content (R2=0.092). Effective results were obtained in terms of activity showing the potential usage of supercritical CO2 extraction to acquire bioactive compounds of interest.

Izvorni jezik
Engleski

Znanstvena područja
Kemija, Kemijsko inženjerstvo, Biotehnologija

POVEZANOST RADA