Naslov
Chromosomal instability and double minute chromosomes in a case of breast cancer patient

Autori
Lalić, Hrvoje ; Volavšek, Črtomir ; Radošević-Stašić, Biserka

Izvornik
Acta Medica Okayama (0386-300X) 58 (2004), 1; 51-58

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
chromosomal aberration in peripheral blood lymphocytes; double minutes; breast carcinoma; radiochemotherapy

Sažetak
Cytogenetic analysis was performed in peripheral blood lymphocytes (PBL) of a woman with ductal breast carcinoma, who as hospital employee was during the 15 years professionally exposed to low doses of ionizing radiation. After the chemotherapy in combination with radiotherapy the most important finding was the presence of double minutes (DM) chromosomes, in combination with other chromosomal abnormalities (on 200 scored metaphases were found 2 chromatid breaks, 10 dicentrics, 11 acentric fragments, 2 gaps and 3 double minutes chromosomes). In repeated analysis (after 6 months) DM chromosomes were still present. To roll out the possibility that she was on work overexposed by ionizing radiation her blood test was compared with the group of coworkers, as well as with a group of professionally unexposed people. The data rejected this possibility, but the retroactive analysis showed that the patient even at the time of employment had moderately increased number of chromosomal aberrations (3, 5%), consisting of 3 isochromatides and 4 gaps, suggesting that initial genomic instability enhanced the development of later events. The finding of continuous presence of rare DM chromosomes in her PBL (4 and 10 months after radio-chemotherapy) was considered as an indicator of additional risk, which might have some prognostic significance.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti, Kliničke medicinske znanosti, Farmacija

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