Naslov
A targeted and untargeted approach to volatile metabolite characterization of "Maraština" wines produced by spontaneous fermentation

Autori
Boban, Ana ; Vrhovsek, Urska ; Carlin, Silvia ; Mucalo, Ana ; Budić-Leto, Irena

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
10th International Congress of Food Technologists, Biotechnologists and Nutritionists - book of abstracts / - Zagreb : Hrvatsko društvo prehrambenih tehnologa, biotehnologa i nutricionista, 2022, 59-60

Skup
10th International Congress of Food Technologists, Biotechnologists and Nutritionists

Mjesto i datum
Zagreb, Hrvatska, 30.11.2022. - 02.12.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
metabolite profiling ; Maraština ; spontaneous fermentation

Sažetak
Spontaneous fermentation in winemaking is a complex microbial reaction that involves influences from many factors, including the microbiota, the grape variety, climatic and soil conditions, and vinification technology. The perceived aroma of the produced wine is the result of interactions between all the volatile and nonvolatile metabolites. The study aimed to investigate the volatile metabolite profile of „Maraština“ wines produced by spontaneous fermentation, thus supporting the concept of terroir impact on the volatile aromas of the wine. The „Maraština“ grapes were harvested in 10 vineyards from two Croatian vine-growing regions: Northern Dalmatia and Central and Southern Dalmatia. A total of 10 experimental fermentation trails in three repetitions were set up on the laboratory scale. Volatile compounds from the wine samples were isolated by solid-phase extraction (SPE) and analyzed by an untargeted approach using two-dimensional gas chromatography coupled with time-of-flight mass spectrometry (GCxGC-TOF-MS) and a targeted approach by gas chromatography-tandem mass spectrometry (GC- MS/MS). Comprehensive two-dimensional GCxGC analysis provided a detailed characterization of total volatile metabolites in the wines due to its excellent separation ability. More than 900 compounds were detected after untargeted profiling and 222 of them were identified or tentatively identified by comparison of experimental retention indices and mass spectra with literature data from the NIST database. A total of 56 volatile compounds were identified and quantified using GC-MS/MS analysis, including terpenes (14), C13- norisoprenoids (3), esters (18), alcohols (4), phenols (4), aldehydes (2), ketones (2), acids (4), lactones (4) and indole (1). The Fischer ratio was useful for establishing the compounds responsible for the main differences between grapes harvested from different subregions. „Maraština“ wines from the Northern Dalmatia subregion had a significantly higher concentration of total volatile compounds than the wines from the Central and Southern Dalmatia subregion, especially the following compounds: cis-Rose oxide, trans-Rose oxide, β-Damascenone, TDN, ethyl leucate, diethyl succinate, phenylacetaldehyde, benzaldehyde and octanoic acid.

Izvorni jezik
Engleski

Znanstvena područja
Prehrambena tehnologija

POVEZANOST RADA