Naslov
Chromosome segregation errors in cells with varying contact levels

Autori
Sošić, Iva ; Risteski, Patrik ; Tolić, Iva

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
5. simpozij studenata doktorskih studija PMF-a : knjiga sažetaka = 5th PhD Student Symposium 2021 : book of abstracts / Barišić, Dajana - Zagreb, 2021, 241-242

ISBN
978-953-607690-1

Skup
Faculty of Science PhD Student Symposium 2021/Simpozij studenata doktorskih studija PMF-a 2021.

Mjesto i datum
Zagreb, Hrvatska, 24.04.2021. - 25.04.2021

Vrsta sudjelovanja
Poster

Vrsta recenzije
Podatak o recenziji nije dostupan

Ključne riječi
Mitotic spindle ; Segregation errors ; Mitotic fidelity ; Cell environment ; Actin

Sažetak
The mitotic spindle is a microtubule-based structure that allows accurate division of genetic material between two daughter cells. Disruption of division mechanisms threaten cell viability or lead to cell transformation if not detected and eliminated by the cell. Improper establishment and maintenance of kinetochore-microtubule attachments can give rise to erroneously attached chromosomes, which lag in anaphase and threaten segregation fidelity [1]. Here, we investigate the importance of cell environment for accurate chromosome segregation in healthy and cancer epithelial cell lines, based on previous research on organoid systems [2]. By using cell micropatterning of U2OS cells, and since recently, both U2OS and MDCKII cells on fibronectin-coated coverslips in varying densities, we determined error rates in isolated cells and cells grown in confluent monolayers. In U2OS cells, chromosome bridges are the most frequent error, while the occurrence of lagging chromosomes is low in both groups of cells. Spindle geometry and interkinetochore distance are not influenced by cellular environment. However, anaphase spindles are less well centered in tightly surrounded cells than in isolated ones, indicating defects in spindle positioning. Cortical actin levels are differently influenced by cell environment depending on the cell type. Future experiments should reveal to what extent and how forces from the cortex are transmitted via astral microtubules to the central spindle and kinetochores.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

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