Naslov
Tyrosine phosphorylation of actin, a sensitive indicator of stress response of coronin-null cells to hyperosmotic shock

Autori
Barišić, Karmela

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
CCOMLIS1, 1st Croatian Congress on Molecula Life Sciences, Opatija, Croatia, June 09-14, 2002, Book of Abstracts / Dumić, Jerka i suradnici - Zagreb : Farmaceutsko-biokemijski fakultet Sveučilišta u Zagrebu, 2002

Skup
CCOMLIS1, 1st Croatian Congress on Molecula Life Sciences

Mjesto i datum
Opatija, Hrvatska, 09.06.2002. - 14.06.2002

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
coronin; Dictyostelium discoideum; tyrosine phosphorylation of actin; hyperosmotic stress

Sažetak
Coronin is a WD-repeat protein involved in cytokinesis, cell motility, and phagocytosis. Here is shown that this actin-binding protein is important for the protection of Dictyostelium cells against osmotic stress. In wild-type cells exposed to hyperosmotic shock of 400 mM sorbitol, coronin localizes to protrusion of the cell surface, where it overlaps with filamentous actin. The viability of coronin-null cells under hyperosmotic stress conditions is dramatically reduced. As a sensitive indicator of stress response in wild-type and coronin-null cells, the tyrosine phosphorylation of actin is used. Tyrosine phosphorylation of actin is strongly increased when coronin-null cells are transferred from starving conditions or growth on bacteria to liquid nutrient medium. This increase is reversible and also observed upon transfer of the cells to 160 mM sorbitol, a non-nutrient solution with the same osmolarity as the liquid medium. A sharp increase of actin phosphorylation has been observed in wild-type development at the onset of sporulation. This increase during fruiting body formation is supposed to be due to water stress conditions, caused by the increase of cytoplasmic osmolyte concentrations.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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