Naslov
Cross-talk between inositol lipid signalling systems localized in renal brush-border and basal-lateral plasma membranes

Autori
Crljen, Vladiana ; Banfić, Hrvoje

Izvornik
Periodicum Biologorum (0031-5362) 105 (2003), 2; 115-118

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
hepatocyte growth factor/scatter factor; endothelin; sarafotoxin S6c; phospholipase C; protein kinase C; phosphoinositide 3-kinase; kidney plasma membranes

Sažetak
Background and Purpose:Majority of hormones acting in the proximal tubule bind to their receptors in basal-lateral plasma membrane (BLM). However, the existane of functionally operated receptors in brush-border plasma (BBM), such as ET_B receptors which activate phospholipase C (PLC), was also demonstrated. The present study was performed to determine a possible existence of cross-talk between signalling systems in BLM and BBM. Materials and Methods: Rat renal cortical BLM were isolated using Percoll gradient method, while BBM were isolated by the Mg^2^+/EGTA-precipitation method. Diacylglycerol (DAG) concentration in BLM and BBM was measured using purified DAG kinase. Results and Conclusion: Cross talk between signalling systems localized in renal BLM and BBM was demonstrated. Sarafotoxin S6c-(S6c) mediated heterologous regulation of hepatocyte growth factor (HGF) signalling in BLM may be mediated by protein kinase C (PKC). On the other hand, HGF-mediated heterologous regulation of S6c signalling in BBM may be mediated by phosphoinositide 3-kinase (PI3K) and/or by one of its downstream signalling events.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA