Naslov
Micronucleus induction in mouse peripheral reticulocytes by 5-nitrofurantoin

Autori
Fučić, Aleksandra ; Marković, Darko ; Ferenčić, Željko

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
From Hazards to Risk : Abstact book / ? - Aberdeen (MD) : European Environmental Mutagen Society, 2003, 49-50

Skup
33rd Annual meeting of European Environmental Mutagen Society

Mjesto i datum
Aberdeen, Ujedinjeno Kraljevstvo, 24.08.2003. - 28.08.2003

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
5-nitrofurantoin; micronucleus; mouse

Sažetak
5-nitrofurantoin (furagin) is used in therapy of urinary infections in adults and children. However, the genotoxicity potential of this compound is still under investigation. According to published data (Slapsyte et al, 2002) the genotoxicity effect in chidlren is detectible after long term application and increased risk of transitional cell carcinoma was reported in patients with history of treatment by this compound. The aim of the current study was to test 5-nitrofurantoin potency to induce micronuclei in mouse reticulocytes. The peripheral blood cells were collectes from tail vein and in vivo micronucleus assay was carried out according to the method of Hayashi et al ( 1990). Animals were divided into three groups (each group was 3 males and 3 females). Concentrations of 10 mg/kg and 50mg/kg were applied to animals. cyclophosphamide at concentration of 75 mg/kg was used as positive control. Animals were sampled before injection and again 48 and 96 h after treatment. The mean value of micronucleus frequency of control samples was 0, 1%. The mean value of positive control was 7, 1%. The incidence of micronucleus frequency at dose of 10 mg/kg was 0, 43% after 48h and 0, 2% after 96h. In animals treated with 50 mg/kg micronucleus frequency was after 48h 0, 46% and after 96h 0, 38%. For both concentrations response peak which significantly deviates from control values eas after 48h. At concentration of 50 mg/kg the micronucleus frequency remains signficantly higher after 96h than at concentration of 10mg/kg suggesting slow rate of elimination of 5-nitrofurantoin and prolonged genotoxic activity. It could be concluded that 5-nitrofurantoin increase significantly micronucleus frequency in mice reticulocytes and that slow rate of elimination could be reason of 5-nitrofurantoin accumulation and risk of genome damage after long term application. According to the described difference in farmacokinetics of furagin in adults and children (Wierzba , 1982) further research should be focused on experiments in young animals.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

Napomena
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