Naslov
The uroguanylin effect on stimulated saliva volume flow, the pH and concentration of Na+, K+ and Cl– in mice

Autori
Jakovac, Domagoj ; Marolt Banek, Iva ; Ratko, Martina ; Lapić, Ivana ; Dugandžić, Aleksandra

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, neobjavljeni rad, znanstveni

Skup
3rd Regional Congress of Physiological Societies and 5th Congress of Croatian physiological Society

Mjesto i datum
NP Plitvička jezera, Hrvatska, 22.09.2022. - 25.09.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
guanylate cyclase C, saliva flow, ion, and water transport

Sažetak
Introduction: The physiological role of signalling pathways for guanylin peptides (GP) has been demonstrated in the kidney and brain, but their unexplored role in the physiology of the salivary glands and their possible role in the regulation of ion secretion not only in physiological but also in pathophysiological conditions, such as cystic fibrosis, Sjoegren's syndrome or oral mucositis, remains to be seen. Aim: This work aimed to determine the effect of uroguanylin (UGN) on the flow volume of stimulated saliva and the pH and concentration of Na+, K+, and Cl- in wild type (WT) animals and animals lacking the guanylate cyclase C (GC-C KO). Materials and methods: A total of 30 wild-type male mice (C57BC/6NCrl), and wild-type mice lacking the GC-C from the same littermates were used. Mice were anesthetized and administered i.p. UGN or physiological solution. After 15 min, pilocarpine was applied to stimulate salivation. Filter paper cut into strips was used to collect saliva. Secreted saliva was determined by the gravimetric method and samples were centrifuged at 15, 000g at 24C for sialochemistry. Concentrations of Na+, K+, and Cl- in saliva were measured by indirect potentiometry, while pH was determined by potentiometry on a blood gas analyser using dedicated pH-sensors. Results: The difference in concentration of Na+, K+ and Cl- after UGN application in saliva of individual groups of animals was not statistically significant. A lower pH was observed in the WT animals upon UGN application, but comparing it to animals lacking GC-C, it was not statistically significant. Salivary flow was reduced during the application of UGN in WT. This effects was missing in same reduction was observed in GC-C WT, whereas animals from the same littermate lacking GC-C were not. Conclusion: Based on the obtained results, we concluded that UGN can affects saliva composition and flow via both GC-C dependent (pH) or GC-C independent (flow) signalling pathway

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA