Naslov
GENETIC STABILITY OF RECOMBINANT VACCINE CANDIDATE F-RSV-MRV2 UNDER SELECTIVE PRESSURE

Autori
Kosutic Gulija, Tanja ; Slovic Anamarija ; Jagusic, Maja ; Pali, Dorotea ; Ivancic Jelecki, Jelena

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
Annual meeting of the Croatian Immunological Society 2022

Mjesto i datum
Sveti Martin na Muri, Hrvatska, 06-08.10.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
genome stability ; RSV vaccine ; recombinant mumps virus ; Vero cell line

Sažetak
Respiratory syncytial virus (RSV) usually causes mild cold-like symptoms, but in infants it can lead to serious diseases like bronchiolitis and pneumonia. Despite great efforts, not a single vaccine is available yet. One of the approaches in the development of RSV vaccines is the preparation of chimeric recombinant viruses expressing RSV glycoprotein (G) or fusion protein (F). Albeit, it has been shown that such constructs can be genetically unstable, resulting in mutation of inserted gene which can reduce or disable expression. We prepared a recombinant virus, F-RSV-MRV2, which contains mumps virus as a vector with inserted FRSV gene. The aim of this research was to investigate genetic stability of F-RSV-MRV2 under selective pressure of two cell lines (Vero and A549) and high and low multiplicity of infection (MOI). Analysis of variability of viral populations was studied by passaging virus stock for 10 passages in triplicate and subsequent sequencing by next-generation sequencing. Our results show that viruses passaged in Vero accumulated mutations in greater numbers and at higher frequencies than viruses passaged in A549. However, all samples had similar number of mutations in the insert. Surprisingly, in all high MOI Vero samples deleterious mutations that would prevent the expression of the F-RSV appeared in the insert. These results show that cell lines have different effects on genetic stability of viruses, while the influence of MOI was not prominent. Also, regular monitoring of the viral genome composition is required during the preparation of recombinant vaccine candidates that are produced in Vero cells.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Biotehnologija, Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

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