Naslov
Can ex vivo magnetic resonance imaging be used to predict sperm presence in testicular tissue of men with azoospermia?
(Can ex vivo magnetic resonance imaging be used to predict sperm presence in esticular tissue of men with azoospermia?)

Autori
Planinić, Ana ; Škokić, Siniša ; Ježek, Davor

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
XXVIth North American Testis Workshop Program book / - , 2022, 33-33

Skup
XXVIth North American Testis Workshop

Mjesto i datum
San Diego (CA), Sjedinjene Američke Države, 04.05.2022. - 07.05.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Azoospermia, Sperm retrieval, Magnetic resonance imaging

Sažetak
Objectives The success rate of TESE in men with azoospermia is around 60% and the only method currently used for sperm retrieval rate prediction is histological analysis, an invasive method that cannot survey the whole testis. The aim of this study was to assess if magnetic resonance imaging (MRI) could be useful in predicting sperm presence in testicular tissue of men with azoospermia. Methods 18 samples of testicular tissue were obtained via TESE. 9 samples underwent MRI as fresh tissue and 9 were frozen in liquid nitrogen, thawed and then imaged. All samples were then histologically analyzed. T1, T2 and ADC values were measured in all samples and values were compared between groups and subgroups. Results The apparent diffusion coefficient (ADC) was increased in fresh and cryopreserved samples without sperm compared to samples that contained sperm while no difference was observed in T2 or T1 values. T2 was increased in cryopreserved compared to fresh samples while ADC and T1 values were consistent. Conclusions ADC, which reflects the diffusion of water, is increased in tissue without sperm due to lower cell density and it could be a useful parameter in predicting sperm presence in fresh and cryopreserved testicular tissue. T2 is significantly increased in frozen and thawed tissue compared to fresh tissue which should be considered when interpreting data and could reflect the effect of freezing and thawing on the consistency and increased diffusion of medium into the tissue. All in all, MRI parameters obtained via ex-vivo imaging of testicular tissue could be useful in predicting sperm presence and evaluation of more parameters is warranted.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA