Naslov
Proteomic Research on the Therapeutic Properties of Medicinal Mushrooms

Autori
Jakopovic, Boris

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, ostalo, znanstveni

Skup
11th International Medicinal Mushroom Conference

Mjesto i datum
Beograd, Srbija, 27-30.09.2022

Vrsta sudjelovanja
Plenarno

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
cancer ; medicinal mushrooms ; proteomics ; bioinformatics

Sažetak
Medicinal mushrooms are increasingly being recognized as an important therapeutic modality in complementary oncology. Until now, more than 800 mushroom species have been known to possess significant pharmacological properties, of which antitumor and immunomodulatory properties have been the most researched. Besides a number of medicinal mushroom preparations being used as dietary supplements and nutraceuticals, several isolates from mushrooms have been used as official antitumor drugs in clinical settings for several decades. Proteomics is a large-scale study of proteins, which is characterized by a hypothesis- free and comprehensive approach to studying novel mechanisms of potential therapeutics. Specifically, differential proteomics, also known as comparative or functional proteomics, studies the changes in proteome in different physiological or pathological states between two or more samples. Cancer proteomics encompasses the identification and quantitative analysis of healthy tissue from neoplasia and can be used to identify markers for cancer diagnosis and treatment (biomarkers), monitoring disease progression, and identifying therapeutic targets serendipitously. Despite its complexity, proteomics is necessary for accurate characterization of pharmacological action. The complexity of cancer, which includes various pathways of its ontogeny and progression, tumor microenvironment, and therapeutic resistance mechanisms, poses demanding challenges that require a systems biology approach that is now increasingly beginning to re-examine the historical reductionist approach. In this regard, the previous research on cancer as well as on cancer therapeutics might be regarded as preliminary or partial and in need of a more in- depth study. However, various challenges remain. Besides the complexity and variability of biological material, i.e., medicinal mushrooms, the variability in the results may also be influenced by the model, either in vitro or in vivo, where the timing of the treatment (early vs. late models of disease ; immunocompetent vs. nude mice xenografts), as well as the tumor model (orthotopic vs. heterotopic), can produce different results. The results are also highly dependent on the proteomic methods, which also differ in their sensitivity. However, these obstacles must be overcome in order to study complex diseases such as cancer.This review is focused on the current state of proteomic research into antitumor mechanisms of some of the most researched medicinal mushroom species, including Phellinus linteus, Ganoderma lucidum, Auricularia auricula, Agrocybe aegerita, Grifola frondosa, and Lentinus edodes, as whole body extracts or various isolates, as well as of complex extract mixtures.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

POVEZANOST RADA