Naslov
Screening bean genotypes for drought resistance using high-throughput phenotyping

Autori
Javornik, Tomislav ; Lazarević, Boris ; Carović- Stanko, Klaudija

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of abstracts of 10th International Congress of Food Technologists, Biotechnologists and Nutritionists / Komes, Drađenka ; Vidaček Filipec, Sanja ; Voučko, Bojana ; Šeremet, Danijela ; Marković, Ksenija ; Rumora Samarin, Ivana / - , 2022, 211-212

Skup
10th International Congress of Food Technologists, Biotechnologists and Nutritionists

Mjesto i datum
Zagreb, Hrvatska, 30.11.2022. - 02.12.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
snimanje klorofilne fluorescencije, multispektralna snimanja, 3D skeniranje, mikrosateliti
(chlorophyll fluorescence imaging, multispectral imaging, 3D scanning, microsatellites)

Sažetak
In the face of rapid climate change, drought is considered the most damaging environmental stress to agricultural production. It is estimated that 60% of beans are grown in regions suffering from water shortages, making drought the biggest problem for its production. The selection of drought-resistant genotypes would be very useful for bean production and the agricultural industry. With advances in modern technology, we can effectively combine knowledge of phenotype and genotype to promote breeding programs. At the University of Zagreb Faculty of Agriculture, samples of traditional bean cultivars from Croatia and surrounding countries are preserved. Traditional cultivars represent valuable genetic material that can be used for the selection of desirable agronomic traits. 200 different bean genotypes will be grown in a growth chamber under controlled conditions, inducing two treatments: Control (well-watered plants) and drought stress treatment. High-throughput phenotyping (HTP) techniques will include chlorophyll fluorescence imaging, multispectral imaging, and 3D scanning. Three measurements will be taken over a period of one week and the occurrence of drought and its effect on plant morphological and physiological traits will be monitored. DNA will be isolated from the leaves and genetic analysis of genotypes will be carried out using Microsatellites (SSR). Single Nucleotide Polymorphisms (SNP) markers will be detected using DartSeq technology. A detailed and in-depth analysis of the physiological background of gas exchange capacity will be performed on the selected tolerant and susceptible bean genotypes, including measurements of photosynthetic rate, transpiration rate, stomatal conductance, intercellular CO2 concentration, water use efficiency, light use efficiency, etc. 212 Association mapping will be carried out to find genetic regions associated with phenotypic parameters of chlorophyll fluorescence, gas exchange parameters, and vegetation indices. This research will help to select potentially resistant bean genotypes to drought stress, which may ultimately contribute to the development of breeding programs and agricultural production itself.

Izvorni jezik
Engleski

Znanstvena područja
Biologija, Poljoprivreda (agronomija), Biotehnologija

POVEZANOST RADA