Naslov
SECOND HEMATOPOIETIC STEM CELL TRANSPLANTATION WITH A DIFFERENT DONOR: IMPLICATIONS FOR CHIMERISM ANALYSIS

Autori
Štingl Janković, Katarina ; Maskalan, Marija ; Burek Kamenarić, Marija ; Serventi-Seiwerth, Ranka ; Duraković, Nadira ; Vrhovac, Radovan ; Žunec, Renata ; Grubić, Zorana

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts / - , 2022, 121-121

Skup
14. Hrvatski biološki kongres

Mjesto i datum
Pula, Hrvatska, 12.10.2022. - 16.10.2022

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Domaća recenzija

Ključne riječi
hematopoietic stem cell transplantation ; chimerism

Sažetak
Chimerism analysis provides valuable information about the efficacy of the hematopoietic stem cell (HSC) transplantation as treatment for various hematologic diseases and disorders. The method of choice for this analysis has recently shifted form short tandem repeat (STR) loci detection towards real-time PCR based methods due to their higher sensitivity. The present study was undertaken in order to evaluate whether the STR analysis should remain a preferred choice in cases when a patient receives a second HSC transplant from a different donor. The study included 17 patients for whom the first transplantation did not achieve favourable outcome, so a second transplantation, with a different donor, was performed. Samples of patient before HSCT, both donors and patient after second HSCT were amplified for 21 STR markers using a commercial kit (Aneufast™ QF-PCR Kit, Genomed Biotech, Harrow, UK). Only the second donor was present in 16 cases, while in the remaining one case, both the first and the second donor were detected in a 36% vs. 64% ratio in the post HSCT sample. This case supports the use of STR markers in chimerism evaluation in all cases when there is a possibility that more than two distinct genotypes are present in tested sample as is the case with second HSCT. As opposed to biallelic insertion/deletion polymorphisms analysed in real- time PCR based protocols, STR markers provide sufficient polymorphism to reliably distinguish all genotypes present in post HSCT sample.

Izvorni jezik
Engleski

POVEZANOST RADA