Pregled bibliografske jedinice broj: 1233028
HPLC methof for determination of natural moisturizing factor components in the stratum corneum
HPLC methof for determination of natural moisturizing factor components in the stratum corneum // 16th International conference, Laboratory competence 2021 Book of abstracts
Brijuni, Hrvatska, 2021. str. 97-97 (poster, međunarodna recenzija, sažetak, znanstveni)
CROSBI ID: 1233028 Za ispravke kontaktirajte CROSBI podršku putem web obrasca
Naslov
HPLC methof for determination of natural moisturizing factor components in the stratum corneum
Autori
Peremin I, Jakasa I
Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni
Izvornik
16th International conference, Laboratory competence 2021 Book of abstracts
/ - , 2021, 97-97
Skup
16th International conference, Laboratory competence 2021
Mjesto i datum
Brijuni, Hrvatska, 13-16.10.2021
Vrsta sudjelovanja
Poster
Vrsta recenzije
Međunarodna recenzija
Ključne riječi
natural moisturizing factor, extraction, liquid chromatography, method validation
(natural moisturizing factor, extraction, liquid chromatography, method validatio)
Sažetak
The levels of natural moisturizing factor (NMF) in the skin can be used as a biomarker of hydration, for studying the effect of skin irritants, or as a biomarker of loss-of-function mutations in the filaggrin gene which are the main risk factor for atopic dermatitis. NMF is a complex mixture of hygroscopic compounds in the uppermost layer of the skin, the stratum corneum (SC) and amino acids and their derivatives contribute more than 50 percent to total content of NMF. Recently, a high-performance liquid chromatography (HPLC) method for the direct determination of four most relevant components of NMF in the SC was proposed based on the extraction with ammonia and subsequent determination by HPLC with mobile phase (MF) containing hydrochloric acid. The main disadvantage of this method is a need to evaporate ammonia to dryness and reconstitute dry content in water, which is time consuming. Another disadvantage is use of hydrochloric acid to sufficiently lower pH of the MF which contribute to corrosion of the chromatographic system. The aim of this study was to explore water as possible substitute for ammonia extraction as well as use of phosphoric acid as substitute of hydrochloric acid. Chromatographic performance, recovery, and precision parameters of the water method was investigated and compared with the extraction using ammonia. In summary, the modified analytical method was shown to be feasible and reliable for the determination of NMF components in the skin.
Izvorni jezik
Engleski
Znanstvena područja
Kemija
