Naslov
First insights into mitogenome variability of indigenous Croatian sheep breeds

Autori
Brajkovic, Vladimir ; Diab, Filip ; Valjak, Nikolina ; Cubric-Curik, Vlatka ; Novosel, Dinko ; Oštarić, Fabijan ; Curik, Ino

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts of the 73rd Annual Meeting of the European Federation of Animal Science / - , 2022, 113-113

Skup
73rd Annual Meeting of the European Federation of Animal Science (EAAP 2022)

Mjesto i datum
Porto, Portugal, 05.09.2022. - 09.09.2022

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Mitogenome ; Croatian sheep breeds ; Haplotypes ; Haplogroups

Sažetak
Sheep rearing is one of the most important types of extensive livestock in Croatia, occurring mainly in coastal and mountainous areas along the eastern Adriatic. We retrieved mitogenome from the whole genome (12× and 30×) of 23 individuals representing eight indigenous sheep breeds (Cres Island Sheep, Dalmatian Pramenka, Dubrovnik Ruda, Istria Sheep, Krk Island Sheep, Lika Sheep, Pag Island Sheep and Rab Island Sheep) and two mouflons. The average coverage of the mitogenome from the 12-fold sequenced genome of 17 samples was 1, 627 (min 322 ; max 9, 588) and from the 30- fold sequenced genome of eight samples was 1, 851 (min 865 ; max 2, 841). Our 16, 624 bp fasta set consisted of 112 parsim-info sites and 247 singleton sites while obtained 24 haplotypes with a haplotype diversity of 0.997. When compared with the mitogenome variability of 347 sheep (86 breeds) and three mouflon (Ovis aries musimon) available in GenBank, the median-joining network showed that 22 haplotypes belonged to haplogroup B and, interestingly, one belonged to haplogroup A and one to haplogroup C. Bayesian analyses also confirmed a high frequency of haplogroup A in the breeds from Central and East Asia, and a high frequency of haplogroup B in Europe and Anatolia. The rare haplogroups C occur in the Iberian Peninsula area, the Middle East, the Caspian Sea region, China, India, and Nepal, while haplogroups D and E may be found in the Middle East. The haplotypes of our mouflons were assigned to haplogroup B along with those of the other European mouflons. The presence of two haplotypes assigned to haplogroups A and C suggests migratory contacts between the Balkans and the Middle East that need to be further explored. In order to clarify the timing of the appearance of A and C haplotypes in the eastern Adriatic, our future analyses will refer to chronological analyses of ancient sheep bones.

Izvorni jezik
Engleski

Znanstvena područja
Poljoprivreda (agronomija), Interdisciplinarne biotehničke znanosti

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