Naslov
Identification of the multidrug resistance-associated protein (MRP) related genes in several marine and freshwater fish species

Autori
Sauerborn, Roberta ; Stupin Polancec, Darija ; Zaja, Roko ; Smital, Tvrtko

Vrsta, podvrsta i kategorija rada
Radovi u zbornicima skupova, cjeloviti rad (in extenso), znanstveni

Izvornik
12th International Symposium on Pollutant Responses in Marine Organisms (PRIMO12) : conference proceedings / - , 2003

Skup
International Symposium on Pollutant Responses in Marine Organisms (12 ; 2O03)

Mjesto i datum
Tampa (FL), Sjedinjene Američke Države, 09.05.2003. - 13.05.2003

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Multixenobiotic Resistance (MXR); P-glycoprotein; MRP-related proteins

Sažetak
Multixenobiotic resistance mechanism (MXR) in aquatic organisms is mediated by the activity of the specific transmembrane P-glycoprotein (Pgp) that binds and actively efflux different chemicals out of cell. In last few years several others, non-Pgp transport proteins have been identified in different human and animal tissues. Concerning their characteristics and tissue distribution there is a reasonable possibility that members of the multidrug resistance-associated protein (MRP) family may also be expressed in aquatic organisms. Therefore, in this study we have tried to identify MRP related genes in different tissues from several marine and freshwater bivalves (Mytilus galloprovincialis, Dreissena polymorpha, Anodonta cygnea) and fish (Mullus barbatus, Cyprinus carpio, Salmo trutta) species. Following an alignment of known MRP1 and MRP2 human sequences, as well as the available mrp2 sequences from animal tissues, we have determined highly conserved regions and used them to design three pairs of consensus primers. Total RNA was isolated, reverse transcribed to cDNA by RT-PCR, and the obtained cDNAs were PCR amplified with the corresponding primers. In the case of cDNAs from liver tissue of all investigated fish species it resulted in three PCR products ranging from 250-269 bp. No PCR products were obtained with cDNAs from other species and/or tissues. The amplified PCR products were sequenced and their homology compared with the available Pgp and MRP protein sequences from different species. Described results clearly pointed at the possibility that at least in fish species MXR as a general defence mechanism may be mediated by the activities of at least two different types of transport proteins.

Izvorni jezik
Engleski

Znanstvena područja
Biologija

POVEZANOST RADA