Naslov
Recording of spontaneous action potentials and electrical stimulation of in-vitro spiral ganglion neurons acquired with high-density neuroelectronic interface
(Recording of spontaneous action potentials and electrical stimulation of in-vitro spiral ganglion neurons acquired with high-density neuroelectronic interface)

Autori
Delipetar, Boris ; Kozina, Tonći ; Žarković, Jelena ; Bedalov, Ana ; Kovačić, Damir

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
Zagreb Bioelectronics Workshop and Croatian Neuroelectronics Symposium 2021

Mjesto i datum
Zagreb, Hrvatska, 4.-6.11.2021

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Domaća recenzija

Ključne riječi
Primary auditory neurons, Microelectrode array, Spontaneous activity, Stimulation

Sažetak
Methods for neural response properties, i.e., triggering action potentials (AP) of spiral ganglion neurons (SGN), are still not thoroughly explored and quantified. We report a new neuroelectronic interface consisting of an operational amplifier, printed circuit boards (PCB) and silicon-based high-density electrode array for this study. The significance of this new interface lays in the capability of performing multiple individual stimulation and recording on 64 electrodes simultaneously. Furthermore, a MATLAB application has been designed and implemented to perform the analysis of acquired data. In this study, a silicon-based chip, morphologically optimized for spiral ganglion neurons ([1], [2]), previously coated with poly-L- ornithine and laminin, was used as a surface for in-vitro growth of neonatal SGN extracted from rat pups. After three and six days in-vitro, respectively (3 and 6 DIV), SGN cultures on silicon-based chips, previously bonded on PCBs, were connected to a neuroelectronic interface, and bursts of spontaneous action potentials were recorded. Also, SGN cultures were stimulated with different current amplitudes, phase durations, and modes of stimulation. Results have shown various types of obtained signals regarding their amplitude, latency, duration, polarity, and repeatability. Some of the obtained spontaneous signals match in some characteristics with previously reported properties of action potential signals. Properties of stimulation responses were also analyzed. These findings are then discussed in light of the potential application of the high- density stimulation-recording setup with the graphene-based substrates.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

POVEZANOST RADA