Naslov
Design and application of high-density neuroelectronic interface for extracellular recording and stimulation of activities of primary auditory neurons cultured in-vitro

Autori
Delipetar, Boris ; Žarković, Jelena ; Kozina, Tonći ; Bedalov, Ana ; Kovačić, Damir

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Skup
BioEL2022

Mjesto i datum
Kirchberg in Tirol, Austrija, 12.-19.03.2022

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
Spiral ganglion neurons, Morphology, Spontaneous activity, Stimulation

Sažetak
Despite notable advances in recent decades in the field of cochlear implants (CI), the neuroanatomical gap has so far proved to be an insurmountable obstacle to further progress. Controlled growth and alignment of spiral ganglion neurons (SGN) stand out as one of the possible approaches to solving this problem, as previous studies have shown that the use of chips with a morphologically optimized surface structure containing micropillars can affect both growth and alignment of SGNs 1-2. However, success in applying this approach requires a better understanding of behavior of SGNs in these conditions. For this purpose, we designed the neuroelectronic interface consisting of an operational amplifier, printed circuit boards (PCB) and silicon-based high-density electrode array with capability of simultaneously recording and stimulating 64 electrodes. Several in-vitro experiments in which the chips, previously coated with poly-L-ornithine and laminin, have been used as a substrate for growth of neonatal SGNs extracted from rat pups, were conducted. Bursts of spontaneous action potentials (AP) were recorded and neurophysiological footprint specific to SGNs was found as proof of presence and functionality of SGNs. Recording sensitivity and amplitude dependence with respect to distance between the electrodes were quantified after the single signal source detection was conducted. Cultures were successfully stimulated with different biphasic pulses of various amplitudes and phase durations. Acquired data were analyzed and visualized in custom MATLAB application. The results are discussed considering the potential use of the high-density stimulation-recording interface combined with graphene-based substrates and its application in cochlear implants.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

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