Naslov
Products of Advanced Glycation in Patients with Type 2 Diabetes and Vascular Disease

Autori
Turk, Zdenka ; Šesto, Mihajlo ; Skodlar, Jasna ; Ferenčak, Goran ; Pokupec, Rajko ; Turk, Nikša ; Stavljenić-Rukavina Ana

Izvornik
Annals of clinical biochemistry (0004-5632) 40 (2003), 5; 552-559

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Coronary artery disease; Diabetes; Advanced glycation; Immune complex

Sažetak
Background. Nonenzymatic glycation leading to advanced glycation endproduct (AGE) formation is thought to contribute to vascular pathology. In the present study, AGEs and anti-AGE antibodies in free and immune complex bound form were assayed in serum of diabetic (n=69) and nondiabetic (n=78) patients with coronary artery disease (CAD), and in control subjects (n=47) free from vascular disease. Methods. Blocking ELISA was used to test immunoreactivity against AGE epitope(s), and competitive ELISA to measure total AGE content. Results. Anti-AGE immunoreactivity was significantly (p=0.045) higher in diabetic than in control subjects. Although wide range of anti-AGE antibody titre was observed in nondiabetic CAD patients, there was no significant difference from control subjects. Both diabetic and nondiabetic CAD patients had a higher concentration of circulating immune complexes containing AGE moiety as antigen than control subjects (DMCAD vs C: p=0.041 ; CAD vs C: p=0.047). Study patients showed positive correlation between serum AGE and AGE-immune complexes (DM: r=0.29, p=0.014 ; CAD: r=0.26, p=0.019), whereas no such correlation was recorded in controls (r=0.08, p=0.89). Conclusion. To our knowledge, this is the first study demonstrating increased AGE- immune complexes in patients with CAD, either with or without diabetes, suggesting that AGE- immune complexes might be involved in the atherosclerotic process, either as the result of it or as part of the pathophysiologic process.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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