Naslov
Natural remedy of modern age – antioxidative and antiproliferative potential of mediterranean plants

Autori
Barišić, Matej ; Vidaković, Josip ; Bellulovich, Ema ; Dadić, Blanka ; Horvat, Paula ; Andrašić, Petra ; Borović, Marko ; Tokić, Matko ; Zovko, Filip ; Štimac, Valentina Dora ; Čeri, Andrea ; Hulina Tomašković, Andrea

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts of the Congress of the Croatian Society of Biochemistry and Molecular Biology HDBMB22: From Science to Knowledge / Dulić, M ; Sinčić, N ; Vrhovac Madunić, I - Zagreb : Hrvatsko Društvo za Biotehnologiju, 2022, 66-66

Skup
Congress of the Croatian Society of Biochemistry and Molecular Biology: From Science to Knowledge (HDBMB22)

Mjesto i datum
Brela, Hrvatska, 28.09.2022. - 01.10.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
mediterranean plants, antioxidative effect, antiproliferative effect, DPPH method, HepG2 cell line, MTS assay, Q-PCR

Sažetak
The aim of this study was to assess the antioxidative and antiproliferative potential of mediterranean plants, Pinus halepensis, Pinus pinea, Pinus brutia, Cichorium intybus, Artemisia annua and Anacyclus clavatus, traditionally used for medicinal purposes. Plant hydrolates were isolated by microwave extraction method from dried plants. Antioxidative properties of the hydrolates were assessed using DPPH method with trolox as a reference compound. The antiproliferative activity of plant hydrolates was examined on HepG2 cell line using the MTS assay. Afterwards, the expression of apoptotic gene BCl2 in HepG2 cells was determined using quantitative PCR after cells were exposed to two different concentrations (20% and 40%) of hydrolates for 24 hours. Each hidrolate, with exception of Artemisia annua showed some antioxidative potential in comparison with trolox. Anacyclus clavatus, Pinus halepensis and Pinus pinea had mild proliferative effect on HepG2 in contrast to Pinus brutia whose hydrolate was cytotoxic. Expression of BCL2 was significantly decreased after HepG2 cells were exposed to 40% hydrolates of Artemisia annua, Anacyclus clavatus and Pinus brutia. Pinus halepensis and Pinus pinea lowered BCL2 expression in both concentrations. Lower expression of BCl2 gene indicates that plant hydrolats have proapoptotic activity. In conclusion, some of these traditional plants could be used as a potential antioxidative and antitumor remedy but their impact on human health should be investigated in more detail in the future.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne tehničke znanosti, Temeljne medicinske znanosti, Farmacija

POVEZANOST RADA