Naslov
Genetic transformation of non-conventional yeasts belonging to the Schwanniomyces genus

Autori
Svetec Miklenić, Marina ; Matanović, Angela ; Slišković, Ana ; Štafa, Anamarija ; Šantek, Božidar ; Svetec, Ivan Krešimir

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, neobjavljeni rad, znanstveni

Skup
7th Croatian Congress of Microbiology with International Participation

Mjesto i datum
Sveti Martin na Muri, Hrvatska, 24.-27.05.2022

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
Schwanniomyces ; non-conventional yeasts ; genetic transformation

Sažetak
The term “non-conventional yeasts” refers to all yeast species other than Saccharomyces cerevisiae and Shizosaccharomyces pombe, the two species which are exceptionally well studied and widely utilized in biotechnological processes. In recent years the interest of researchers and industry steers towards non-conventional yeasts harbouring new and potentially exploitable metabolic pathways or other features favourable in biotechnological processes such as thermotolerance, halotolerance, resistance to various growth inhibitors etc. These yeasts are being investigated both as sources of interesting genes which can be transferred into conventional systems but also as the industrial work organisms themselves. In the latter case it is desirable that the work organism can be genetically transformed and even precisely genetically modified allowing construction of improved and more versatile industrial strains. The Schwanniomyces genus is comprised of seven species of budding yeasts which are fairly poorly explored but could have interesting biotechnological properties. Notably, most species form this genus can grow on D-xylose and cellobiose making them worth exploring in the context of production of valuable biochemicals on waste lignocellulose materials as a substrate. Furthermore, the most prominent member of the genus, S. occidentalis var. occidentalis, is potentially interesting producer of heterologous proteins. The aim of this work was to establish a set of basic genetic tools allowing genetic transformation and construction of replicative plasmids for yeasts belonging to the Schwanniomyces genus, thus facilitating further research on these yeasts as well as their industrial application. Using an electroporation protocol, we developed earlier for Brettamomyces bruxellensis several species form the Schwanniomyces genus were successfully transformed with fairly high transformation efficiency. Moreover, several plasmid replication origins belonging to various budding yeast species were tested and origin belonging to a closely related species Scheffersomyces stipitis was identified to be active in Schwanniomyces yeasts, allowing construction of completely stable replicative plasmid in certain Schwanniomyces species.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

POVEZANOST RADA