Naslov
Introduction of p16Ink4a biomarker on fresh and archival cervical smear

Autori
Audy-Jurković, Silvana ; Ovanin-Rakić, Ana ; Jurič, Danijela ; Babić, Damir

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
3. Hrvatski kongres kliničke citologije s međunarodnim sudjelovanjem knjiga sažetaka / Jonjić, Nives ; Kardum.Skelin, Ika - Karlovac, 2005, 175-175

Skup
3. Hrvatski kongres kliničke citologije s međunarodnim sudjelovanjem

Mjesto i datum
Opatija, Hrvatska, 08.05.2005. - 11.05.2005

Vrsta sudjelovanja
Poster

Vrsta recenzije
Domaća recenzija

Ključne riječi
p16INK4a, biomarker

Sažetak
Aim of the study. To introduce immunocytochemical visualization of p16INK4a biomarker on fresh and archival cervical smears. Method. p16INK4a is a specific inhibitor of the cycline-dependent kinase (CDK4 and CDK6) which plays a crucial role in the regulation of the cell cycle by retinoblastoma protein phosphorylation. Increased expression of the high- risk human papillomavirus E6 and E7 oncogenes through binding to the retinoblastoma protein and release of transcription factor E2F results in a highly specific increase in p16INK4a protein expression in dysplastic and malignant cells of squamous and cylindric epithelium of the cervix, which is detectable by a specific monoclonal antibody. The criteria for patient selection were cytologic and histologic diagnosis of cervical intraepithelial neoplasia (CIN) II and III, and adenocarcinoma in situ (AIS). The expression of p16INK4a was immunocytochemically visualized in fresh fixed cervical smears and reprocessed conventional Papanicolaou stained slides. A fresh exocervical sample was in part smeared onto the slide, and in part placed in saline according to our own modification (Ovanin-Rakiæ) and centrifuged. Reprocessing of archival slides was performed according to Choi (1991). The specific biomarker was visualized by use of DakoCytomation p16INK4a Cytology Kit containing specific anti-mouse monoclonal antibody (clone E6H4) and EnVision visualization system. Results. Test result is considered positive if brownish granules are found in the nuclei and/or cytoplasm of at least five dysplastic or malignant cells. The number of positive cells and intensity of nucleus and/or cytoplasm staining vary from case to case. Conclusion. In future studies, the immunocytochemical analysis of p16INK4a expression will be applied in a greater number of cervical smears to evaluate the method in clinical setting.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

POVEZANOST RADA