Naslov
MORPHOMETRIC NUCLEAR IMAGE ANALYSIS IN ENDOMETRIAL CYTOLOGIC SAMPLES

Autori
Mahovlić, Vesna ; Audy-Jurković, Silvana ; Ovanin- Rakić, Ana ; Škopljanac-Mačina, Lada ; Barišić, Ana ; Ilić-Forko, Jadranka ; Škrablin-Kućić, Snježana, Božikov, Jadranka

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
3. HRVATSKI KONGRES PATOLOGIJE I SUDSKE MEDICINE 3. HRVATSKI KONGRES KLINIČKE CITOLOGIJE 1. HRVATSKI SIMPOZIJ CITOTEHNOLOGIJE s meðunarodnim sudjelovanjem Knjiga sažetaka / Jonjić, Nives ; Kardum.Skelin, Ika - Karlovac, 2005, 150-150

Skup
3. HRVATSKI KONGRES PATOLOGIJE I SUDSKE MEDICINE 3. HRVATSKI KONGRES KLINIÈKE CITOLOGIJE 1. HRVATSKI SIMPOZIJ CITOTEHNOLOGIJE s meðunarodnim sudjelovanjem

Mjesto i datum
Opatija, Hrvatska, 08.05.2005. - 11.05.2005

Vrsta sudjelovanja
Predavanje

Vrsta recenzije
Domaća recenzija

Ključne riječi
morphometric nuclear image analysis, endometrium cytology

Sažetak
Aim of the study. To determine morphometric variables and nuclear DNA content in endometrial cytologic samples by use of image analysis. Patients and Methods. Feulgen method of staining (Merck, Germany) was used on May- Grünwald-Giemsa stained archival aspiration cytology endometrium specimens with the following histopathologic diagnoses: proliferation, hyperplasia simplex, hyperplasia complex, hyperplasia complex atypica, and adenocarcinoma endometrii grade I (n=5 each). Image analysis was performed by use of the Sform (Vamstec, Zagreb) software. The image (x100 oil) was transferred from a microscope (Olympus BHS) via CCD TV color camera (Sony) to a PC by digitalized image. Integrated optical density (IOD) as a measure of nuclear DNA content was calculated for each nucleus (n$100). Lymphocytes (n$20) were used as internal control, along with the factor of correction. The morphometric parameters of nuclear area, nuclear perimeter, and factors of nuclear regularity and nuclear ellipticity were analyzed. Statistical analysis was done by use of Statistica ver. 6.0. Results. Multivariate analysis (ANOVA) yielded significant differences (p<0.01) in nuclear DNA content (IOD) and all morphometric variables among normal, hyperplastic and malignant endometrium. On group comparison (Scheffe test), the factors of nuclear regularity (form factor) and nuclear ellipticity distinguished only well differentiated endometrial adenocarcinomas according to proliferation and type of endometrial hyperplasia (p<0.05), whereas the parameters of nuclear area, nuclear perimeter and nuclear DNA content (IOD) showed statistical significance (p<0.05) in all groups. Conclusion. Morphometric variables and nuclear DNA content (IOD) can provide useful information in the cytologic analysis of normal, hyperplastic and malignant endometrium.

Izvorni jezik
Engleski

Znanstvena područja
Kliničke medicinske znanosti

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