Naslov
Lipid production from lignocellulosic biomass by yeast Trichosporon oleaginosus

Autori
Ivančić Šantek, Mirela ; Bakija, Ivana ; Beluhan, Sunčica ; Šantek, Božidar

Vrsta, podvrsta i kategorija rada
Sažeci sa skupova, sažetak, znanstveni

Izvornik
Book of Abstracts: Natural resources green technology & sustainable development/2 / Radojčić Redovniković, Ivana ; Radošević, Kristina ; Jakovljević, Tamara ; Stojaković, Renata ; Gaurina Srček, Višnja ; Erdec Hendrih, Dina - Zagreb : Prehrambeno-biotehnološki fakultet Sveučilišta u Zagrebu, 2016, 97-97

ISBN
978-953-6893-03-4

Skup
Natural resources green technology & sustainable development/2

Mjesto i datum
Zagreb, Hrvatska, 05.10.2016. - 07.10.2016

Vrsta sudjelovanja
Poster

Vrsta recenzije
Međunarodna recenzija

Ključne riječi
biodiesel ; lignocellulose ; microbial lipids ; oleaginous microorganisms

Sažetak
Biodiesel is a renewable substitute fuel for traditional petroleum-based diesel. It consists of the methyl esters of the fatty acids produced by trans-esterification of triglycerides with an alcohol such as methanol under mild conditions in the presence of a base catalyst Main feedstocks used for commercial production of first generation biodiesel are plant oils(rapeseed, soybean. palm, sunflower), animal fats and waste oils. Compared to petroleum diesel, biodiesel is less toxic, biodegradable and does not contain sulphur or aromatics Despite these benefits, the major obstacle to widespread commercialization of biodiesel production is the high production cost relative to petroleum diesel. The high price of the feedstock is the major factor determining the biodiesel price. Intensive search for alternative feedstock brought back in focus the microbial lipids. An oleaginous microorganisms have ability to accumulate significant amounts of lipids (>20% of dry matter) under certain cultivation conditions. Production costs could be significantly reduced by replacing expensive carbon sources (e.g. glucose) with low-value substrates such as agricultural wastes, food waste streams and industrial by-products. In this work, oleaginous yeast Trichosporon o!eaginosus was used for the production of lipids from corn cobs hydrolysate Batch process of simultaneous saccharification and lipid production (SSLP) was conducted at different initial substrate loadings (5-20 % w/w) The highest final lipid yield of 92.4 mg lipid per g of dry matter of pretreated corn cobs (g/gDM_PCC) was obtained at substrate loading of 125% (w/w). A "fed-batch" strategy of sequential loading of substrate during SSLP process was investigated to achieve high cumulative substrate loadings, reduce viscosity of lignocellulosic slurry and obtain higher lipid yields. Batch additions of 2.5 and 5% (w/w) substrate resulted in cumulative substrate loading of 20% (w/w). The strategy of gradual substrate addition was related to the homogenous medium conditions during bioprocess due to the more efficient mixing process Fed batch process of SSLP with substrate additions of 2.5 % (w/w) significantly increased the lipid yield in comparison to the batch process Maximal lipid yield of 269.25 mg/ gDM_PCC was obtained after 312 hours of cultivation.

Izvorni jezik
Engleski

Znanstvena područja
Biotehnologija

POVEZANOST RADA