Naslov
Izraženost LRP1 receptora u hipokampalnoj formaciji zdravih osoba i osoba oboljelih od Alzheimerove bolesti
(Expression of LRP1 receptor in the hippocampal formation in healthy and subjects with Alzheimer's disease)

Autori
Štampar, Emma

Vrsta, podvrsta i kategorija rada
Ocjenski radovi, diplomski rad, diplomski

Fakultet
Prehrambeno-biotehnološki fakultet

Mjesto
Zagreb, Hrvatska

Datum
23.09

Godina
2022

Stranica
46

Mentor
Šver Lidija ; Šimić, Goran

Neposredni voditelj
Španić, Ena

Ključne riječi
LRP1 protein ; Alzheimerova bolest ; hipokampalna formacija ; tau protein ; imunofluorescencija
(LRP1 protein ; Alzheimer's disease ; hippocampal formation ; tau protein ; immunofluorescence)

Sažetak
In recent years, accumulated evidence suggests the involvement of the LRP1 protein in the pathogenesis of Alzheimer's disease (AD), the most common primary cause of dementia syndrome. The LRP1 protein is a member of the LDL receptor family with multiple roles, of which the potentially most important in the mediation of cholesterol entry into cells, especially neurons, and many other roles are related to the metabolism of amyloid precursor protein (APP), whereas some relate to tau protein signaling pathways. Therefore, the purpose of this research was to examine the expression of LRP1 protein in the hippocampus of persons who suffered from AD and persons who died due to other causes and did not have neurological or psychiatric disorders. In other words, we tested whether increased or decreased expression of LRP1 is associated with neuropathological changes characteristic of AD, especially with neurofibrillary degeneration. The results of the analysis of six postmortem tissue samples of the hippocampus (three from subjects with AD and three from control subjects) were obtained by the simultaneous visualization of LRP1 protein and tau protein phosphorylated on the Ser202/205 epitope using the AT8 antibody and showed increased LRP1 protein levels in AD patients compared to control samples. The greater levels of neurofibrillary changes assessed by neuronal AT8-immunoreactivity negatively correlated with the LRP1 protein expression levels. This preliminary result confirms the initial hypothesis on the involvement of the LRP1 protein in the development of AD, but for a firm conclusion, it would be necessary to analyze a much larger number of samples in different stages of the disease.

Izvorni jezik
Hrvatski

Znanstvena područja
Biologija, Temeljne medicinske znanosti, Kliničke medicinske znanosti, Kognitivna znanost (prirodne, tehničke, biomedicina i zdravstvo, društvene i humanističke znanosti), Biotehnologija u biomedicini (prirodno područje, biomedicina i zdravstvo, biotehničko područje)

POVEZANOST RADA