Naslov
CYTOTOXIC EFFECT OF THIENO[2,3-B]PYRIDINE DERIVATIVES ON HUMAN BLADDER CANCER CELLS
(Citotoksično djelovanje tieno[2,3-b]piridinskih spojeva na stanice humanog karcinoma mokraćnog mjehura)

Autori
Kalezic, Denis

Vrsta, podvrsta i kategorija rada
Ocjenski radovi, diplomski rad, diplomski

Fakultet
Medicinski fakultet

Mjesto
Split

Datum
15.09

Godina
2022

Stranica
49

Mentor
Čikeš Čulić, Vedrana

Neposredni voditelj
Vedrana Čikeš Čulić

Ključne riječi
MTT, bladder cancer, thieno-pyridine
(MTT, karcinom mokraćnog mjehure, tienopiridin)

Sažetak
Objectives: The purpose of this study was to determine the effects of treating bladder cancer cells with the newly synthesized thieno[2, 3- b]pyridine anticancer agents, by focusing on its cytotoxic effects on the investigated human cell line T24. Methods: The T24 bladder cancer cell line was treated with a newly developed thieno[2, 3- b]pyridine anticancer compounds: Inhibitor 5 3- amino-N-(3-chloro-2-methylphenyl)-5-oxo-5, 6, 7, 8- tetrahydrothieno[2, 3-b]quinoline-2-carboxamide, Inhibitor 6 3-amino-N-(naphthalen-1-yl)-5-oxo- 5, 6, 7, 8-tetrahydrothieno[2, 3-b]quinoline-2- carboxamide, Inhibitor 8 (E)-3-amino-5-(3-(3- bromophenyl)acryloyl)-N-(3-chloro-2- methylphenyl)-6-methylthieno[2, 3-b]pyridine-2- carboxamide and Inhibitor 9 (E)-3-amino-5-(3-(3- bromophenyl)-1-hydroxyallyl)-N-(3-chloro-2- methylphenyl)-6-methylthieno[2, 3-b]pyridine-2- carboxamide to determine its cytotoxic effect on T24 cells. The 3-(4, 5-dimethylthiazolyl-2)-2, 5- diphenyltetrazolium bromide (MTT) assay was performed to analyze the cellular metabolic activity and determine the cytotoxic effect. Results: Inhibitors 5, 6, 8 and 9 all showed significant cytotoxic effect on the bladder cancer cells in a dose- and time-dependent manner. The most effective was Inhibitor 8 with an IC50 value of 0.4041 µg/mL after 72 hours. Conclusion: Due to their cytotoxic effect on bladder cancer cells, Inhibitors 5, 6, 8 and 9 deserve further attention as a potential treatment for transitional cell cancer.

Izvorni jezik
Engleski

Znanstvena područja
Temeljne medicinske znanosti

POVEZANOST RADA